The aim of this study is to investigate the effects of the combination of sea cucumber peptide and plateau medicinal and edible raw materials (yak whip,deer blood,maca) on the reproductive health of male mice with exercise fatigue.The male model of exercise fatigue was established by acute exhaustion swimming,and the mice were randomly divided into blank group,model group,control group (0.5 mg/g BW commercially available Renshen Duzhong tablets) and two-dose composition group (0.25 and 0.5 mg/g BW formula),which were administered continuously for six weeks.The organ coefficient,mating ability,fatigue resistance index,tissue section,serum hormone level,sperm morphology and malformation rate of mice were measured.Both dosage formula improved the decreased mating ability caused by exhaustive exercise,and the low-dose group has better effects.Also,the swimming time of mice was prolonged by formula treatment,while the level of lactic acid in serum and malondialdehyde in liver were reduced.Low and high-dose formula could effectively improve insufficient serum testosterone and luteinizing hormone levels caused by exhaustive exercise,and reduce sperm deformity rates (P <0.05).In addition,testosterone,lactic acid and malondialdehyde levels were significantly correlated with the mating behavior of mice.In the exercise-induced fatigue mouse model,the combination of sea cucumber peptide and plateau medicinal and edible raw materials can improve the fatigue symptom and hormone secretion imbalance of mice,as well as reduce oxidative damage to the reproductive system.