Rosa roxburghii Tratt.(RRT)is a valuable medicinal and edible plant resource.Investigating the distribution differences of flavonoid compounds in its various medicinal parts provides a theoretical basis for the further development and utilization of RRT.This study focused on Guizhou RRT and employed non-targeted metabolomics combined with principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) to analyze the metabolic differences among its root,leaf,and fruit.In this study,the UPLC-MS technology was adopted,combined with metabolomics analysis methods,to conduct qualitative and quantitative analysis of flavonoids in the root,leaf and fruit of RRT.A total of 3 601 metabolites were detected,among which 165,192 and 180 flavonoids were identified in the root,leaf and fruit respectively,covering 9 types such as chalcones,flavones,flavonols and anthocyanins.The PCA results showed that the three groups of samples were significantly separated,indicating significant differences in metabolites among different parts.The analysis of differential metabolites (fold change≥ 2 or ≤ 0.5,variable importance in projection value≥ 1) indicated that there were 110,118 and 82 differential flavonoid metabolites between leaf and fruit,leaf and root,and fruit and root respectively.KEGG enrichment analysis showed that the differential metabolites were mainly involved in the flavonoid biosynthesis pathway (with the highest proportion of metabolites,reaching 6-7 per group),indicating that their distribution was closely related to the organ-specific functions.In conclusion,this study systematically revealed the characteristics of flavonoids in different medicinal parts of RRT,providing a theoretical basis for the development of medicinal resources.