天然产物研究与开发 ›› 2025, Vol. 37 ›› Issue (12): 2284-2292.doi: 10.16333/j.1001-6880.2025.12.012 cstr: 32307.14.1001-6880.2025.12.012

• 开发研究 • 上一篇    下一篇

基于HPLC指纹图谱及竹节参皂苷IVa含量探讨白扁豆和扁豆衣的药用价值

刘  畅1,2，袁  丁1,2,3，张继红3，黄  玲1,2*，何毓敏1,2,3*   

  1. 1三峡大学国家中医药管理局药理科研三级实验室；2三峡大学健康医学院，宜昌 443002；3湖北省功能性消化系统疾病中医临床医学研究中心，宜昌443199
  • 出版日期:2025-12-30 发布日期:2025-12-29
  • 基金资助:
    湖北森元药业有限公司横向课题（SDHZ2022068）

Medicinal value of Lablab Semen Album and its seed coat based on HPLC fingerprint and chikusetsusaponin IVa content analysis

LIU Chang1,2,YUAN Ding1,2,3,ZHANG Ji-hong3,HUANG Ling1,2*,HE Yu-min1,2,3*   

  1. 1Third-grade Pharmacological Laboratory on Traditional Chinese Medicine,State Administration of Traditional Chinese Medicine,China Three Gorges University;2College of Medicine and Health Sciences,China Three Gorges University,Yichang 443002,China;3Hubei Province Clinical Research Center for Functional Gastrointestinal Diseases of Traditional Chinese Medicine,Yichang 443199,China
  • Online:2025-12-30 Published:2025-12-29

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摘要：

建立白扁豆和扁豆衣的指纹图谱及竹节参皂苷IVa（chikusetsusaponin IVa，CS-IVa）的含量测定方法，并与黑扁豆、红扁豆及其他17种豆类进行比较，以探究其药用价值。采用高效液相色谱法（HPLC）建立特征图谱，并通过化学计量法进行聚类分析（hierarchical cluster analysis，HCA）、主成分分析（principal component analysis，PCA）和正交偏最小二乘判别分析（orthogonal partial least squares discriminant analysis，OPLS-DA）对不同种类和部位的扁豆及其他豆类进行化学模式识别分析。结果表明，白扁豆和扁豆衣的HPLC特征图谱确定了14个共有峰，其中2个为共有色谱峰（原儿茶酸、大豆苷），2个为差异特征峰（染料木苷、CS-IVa）。HCA将15批样品分为2类，OPLS-DA分析筛选出7个影响质量的共有特征峰，CS-IVa被确定为定量分析指标，且主要集中在种皮部。研究结果表明，白扁豆和扁豆衣在HPLC特征图谱和CS-IVa含量上与其他常见豆类有明显区别，为科学阐述其药用价值提供了依据。

关键词: 白扁豆, 竹节参皂苷IVa, 高效液相色谱法, 指纹图谱, 化学计量学分析, 含量测定

Abstract:

This study aimed to establish the HPLC fingerprint profiles of Lablab Semen Album (LSA) and its seed coat (LSAC),and the content determination method for chikusetsusaponin IVa (CS-IVa),and compare them with Dolichos lablab seed with black seed coat (black lentils) and with red seed coat (red lentils),as well as 17 other types of beans to explore their medicinal value.HPLC was used to establish the characteristic profiles,and chemometric methods including hierarchical cluster analysis (HCA),principal component analysis (PCA),orthogonal partial least squares discriminant analysis (OPLS-DA) were applied to perform chemical pattern recognition analysis on different types and parts of lentils and other beans.The results showed that the HPLC fingerprint profiles of LSA and LSAC were established with 14 common peaks identified,including two common chromatic peaks (protocatechuic acid and soyasaponin) and two differential characteristic peaks (genistin and CS-IVa).HCA classified 15 batches of samples into two categories,and OPLS-DA analysis screened out seven common characteristic peaks affecting quality.CS-IVa was determined as the quantitative analysis indicator and was mainly concentrated in the seed coat.The study results indicated that LSA and LSAC were clearly distinguished from other common beans in terms of HPLC fingerprint profiles and CS-IVa content,providing a scientific basis for elucidating their medicinal value.

Key words: Lablab Semen Album, chikusetsusaponin IVa, high-performance liquid chromatography, fingerprinting, chemometric analysis, content determination

中图分类号:  R284.1

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