This study aims to explore the blood-activating and stasis-resolving effects of wine-processed Angelicae Sinensis Radix (WP-ASR) on rats with acute blood stasis model and reveal underlying mechanism.After continuous intragastric administration with aqueous decoctions of Angelicae Sinensis Radix (ASR) and WP-ASR for seven days,an acute blood stasis model was established using a compound method involving ice-water bath immersion combined with subcutaneous injection of epinephrine hydrochloride (0.8 mg/kg).The blood-activating and stasis-resolving efficacy of WP-ASR was evaluated by measuring hemorheological parameters,prothrombin time,activated partial thromboplastin time,thrombin time,and fibrinogen levels.Furthermore,LC-MS/MS non-targeted metabolomics technology coupled with multivariate statistical analysis was employed to compare endogenous metabolites in rat serum across groups.Differential metabolites were screened,and their associated metabolic pathways were constructed.The results showed that ASR and WP-ASR could significantly improve the hemorheological characteristics of the acute blood stasis rat model,prolong prothrombin time,activated partial thromboplastin time,thrombin time and reduce fibrinogen content.Metabolomics analysis showed that 113 differential metabolites were closely related to the blood stasis syndrome,among which 18 differential metabolites were significantly attenuated after ASR intervention,mainly involving linoleic acid metabolism and unsaturated fatty acid biosynthesis.Twenty-two differential metabolites were significantly attenuated after WP-ASR intervention,regulating retinol metabolism,linoleic acid metabolism,arachidonic acid metabolism,and phenylalanine,tyrosine,and tryptophan biosynthesis.In summary,the effects of WP-ASR on acute blood stasis model rat are better than those of ASR,and its mechanism may be related to regulating retinol metabolism,arachidonic acid metabolism and tryptophan metabolism.