关键词: 玫瑰黄链霉菌, OSMAC方法, 次级代谢产物, 抗肿瘤活性

Abstract: In this study,secondary metabolites of Streptomyces roseofulvus M63 were obtained under four fermentation ways using OSMAC methods,and their antitumor activity was examined.Compounds were firstly isolated and purified by column chromatography on Silica gel,Silica gel thin layer chromatography, Sephadex LH-20,reverse phase silica gel and preparative chromatography.The structures of purified compounds were identified by NMR and MS.Their antitumor activity was eventually evaluated using MTT method.The results showed that,fourteen compounds were purified and identified as p-cresol(1),4-hydroxyphenyl ethanol (2),2,3,4,5-tetra hydroxy,methyl ester(3),3,4-dihydroxy benzoic acid(4),4-hydroxy benzoic acid(5),1H-Pyrrole-2-carboxylic acid(6),(22E,24R)-Ergosta-7,22-dien-3β-ol (7),4-hydroxy benzaldehyde (8),3β-hydroxy-5α,3β-hydroxy-5α,8α-ergosterol peroxide-6,22-diene(9),4-hydroxy phenylacetic (10),5-(2-Methylphenyl)-4-penten oic acid(11),3β-hydroxy-ergosterol-5,7,22-triene(12),(5-hydroxy-2-oxo-2H-pyran-4-yl) acetate (13),(4S,5S,9aR)-5-(E)-4carboxy methyl-8-en)-10,13-dimethyl-6- deca hydrronaphthalene-carboxylic acid(14).It was the first time to obtainthese compounds from Streptomyces roseofulvus M63,cervical cancer (HeLa) cells and ovarian cancer(Skov3)were inhibited by compound 11,13 for different degrees.The IC₅₀ values of compound 11 was 38.26 and 14.25 μmol/mLthat less thanpositive control cisplatin only at 24h.The IC₅₀ values of compound 13 on HeLa cells and Skov3 cells was 25.62,11.34,6.524 and 7.892,10.32,5.671,at 24 h,48 h and 72 h,respectively.

Key words: Streptomyces roseofulvus, OSMAC method, secondary metabolites, anti-tumor activity