天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (12): 2056-2062.doi: 10.16333/j.1001-6880.2022.12.009

• 研究简报 • 上一篇    下一篇

剑叶龙血树内生真菌Colletotrichum sp.B-152次生代谢产物研究

张科涛，黄之镨，赵玉瑛，张   冰，许小蓉，许玉潇，赵   庆，张晓梅*   

  1. 云南中医药大学基础医学院&中药学院，昆明 650500
  • 出版日期:2022-12-28 发布日期:2022-12-29
  • 基金资助:
    国家自然科学基金（21662048）；云南省万人计划“青年拔尖人才”专项（YNWR-QNBJ-2019-161）

Study on secondary metabolites of Colletotrichum sp.B-152 derived from Dracaena cochinchinensis

ZHANG Ke-tao,HUANG Zhi-pu,ZHAO Yu-ying,ZHANG Bing,XU Xiao-rong,XU Yu-xiao,ZHAO Qing,ZHANG Xiao-mei*   

  1. College of Basic Medicine & College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine,Kunming 650500,China
  • Online:2022-12-28 Published:2022-12-29

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摘要：

采用色谱技术和波谱学分析从剑叶龙血树内生真菌Colletotrichum sp.B-152的培养液和菌丝中，鉴定出了9个化合物：2-hydroxy-4-methyl-1-phenyl-3-pentanone（1）、4-methyl-1-phenyl-2,3-pentanediol（2）、胸腺嘧啶核苷（3）、腺嘌呤核苷（4）、(22E,24R)-ergosta-7,22-diene-3β,5α,6β-triol（5）、(4E,8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glycopyranosyl-9-methyl-4,8-sphingadienine（6）、tuberosider（7）、 (22E,24R)-5α,8α-epidioxyergosta-6,22-dien-3β-ol（8）、十七烷酸（9），其中化合物1作为天然产物首次报道，化合物2~4、6、7、9首次从刺盘孢属Colletotrichum真菌中分离得到。采用滤纸片扩散法对单体化合物的抑菌活性进行测定，9个化合物对至少一种指示菌表现出不同程度的抑制作用，其中化合物7和8表现出较为广谱的抑菌活性。

关键词: 剑叶龙血树, 内生真菌, 刺盘孢属, 次级代谢产物, 抑菌活性

Abstract:

Nine compounds were isolated and identified from the fermentation of Colletotrichum sp.B-152,which derived from Dracaena cochinchinensis, by chromatographic and spectroscopic analysis.They are 2-hydroxy-4-methyl-1-phenyl-3-pentanone (1),4-methyl-1-phenyl-2,3-pentanediol (2),thymidine (3),adenosine (4),(22E,24R)-ergosta-7,22-diene-3β,5α,6β-triol（5）,(4E,8E)-N-D-2′-hydroxypalmitoyl-1-O-β-D-glycopyranosyl-9-methyl-4,8-sphingadienine（6）,tuberosider（7）, (22E,24R)-5α,8α-epidioxyergosta-6,22-dien-3β-ol（8）,daturic acid (9).Among which,2-hydroxy-4-methyl-1-phenyl-3-pentanone (1) was reported as natural product for the first time,compounds 2-4,6,7 and 9 were isolated from genus Colletotrichum for the first time.The antimicrobial activities of isolated compounds were determined by paper diffusion method.These 9 compounds showed inhibition abilities against more than one pathogens,compounds 7 and 8 showed broad-spectrum antimicrobial activities.

Key words: Dracaena cochinchinensis, endophytic, Colletotrichum sp., secondary metabolites, antimicrobial activities

中图分类号:  R284.2

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