天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (4): 629-633.doi: 10.16333/j.1001-6880.2018.4.016

• 研究简报 • 上一篇    下一篇

蕨菜乙醇提取物降尿酸作用机理及肾保护作用研究

谷仿丽,韩邦兴*,陈乃富,赵颂   

  1. 皖西学院生物与制药工程学院,六安 237012
  • 出版日期:2018-05-07 发布日期:2018-05-07
  • 基金资助:

    安徽省自然科学基金(1508085KH203);安徽省优秀青年人才支持计划;安徽省大学生创新创业训练计划(AH201410376002)

Mechanism of Lowering Uric Acid and Renoprotective Function of Ethanol Extract from Pteridium aquilinum in Hyperuricemia Mice

GU Fang-li, HAN Bang-xing*, CHEN Nai-fu, ZHAO Song   

  1. College of Biological and Pharmaceutical Engineering,West Anhui University,Lu′an 237012,China
  • Online:2018-05-07 Published:2018-05-07

摘要: 为研究蕨菜乙醇提取物降尿酸作用机理及其肾保护作用,将蕨菜乙醇提取物灌胃模型小鼠1周,于给药第3 d,氧嗪酸钾灌胃复制高尿酸血症小鼠模型,检测血清尿酸水平、血及肝脏黄嘌呤氧化酶活性,探讨其治疗高尿酸血症作用机理;蕨菜乙醇提取物灌胃模型小鼠1周,检测血Cr与BNU、肾组织NO与ET水平、肾组织形态学,考察其肾保护作用。结果发现蕨菜乙醇提取物可显著降低小鼠血清尿酸水平,对小鼠血及肝脏黄嘌呤氧化酶活性无显著影响,显著降低血Cr、血BNU、肾组织ET水平,升高肾组织NO水平,肾组织形态学正常。结果表明蕨菜乙醇提取物具有降尿酸及肾保护作用,其降尿酸作用机制不在影响黄嘌呤氧化酶活性,确切的作用机制还有待于进一步研究。

关键词: 蕨菜, 乙醇提取物, 高尿酸血症, 尿酸, 黄嘌呤氧化酶, 肾脏

Abstract: To study the effect and mechanism of the ethanol extract from Pteridium aquilinum on lowering uric acid and renoprotective function,the mice model of hyperuricemia was established by oxygen hydrochloride acid potassium salt.The ethanol extracts of P.aquilinum were continuously i.g. administered once a day to the mouse model for one week.One hour after the final treatment,the urate levels in serum and the hepatic xanthine oxidase (XOD) activity in serum and liver were tested to explore the mechanism of anti-hyperuricemia.After the model mice were i.g. administered with the ethanol extracts of P.aquilinum for one week,the creatinine (Cr) and urea nitrogen (BUN) levels in their serum,as well as the NO,and ET levels in their renal tissue and the histopathology of kidney were tested to investigate the effect on renal function.The results indicated that P.aquilinum ethanol extract can significantly lower the urate and Cr levels in the serum and the BNU and ET in renal tissue of model mice,respectively.P.aquilinum ethanol extract can also elevate the NO levels in renal tissue while showed no effect on the kidney histopathology.Hence,it was concluded that P.aquilinum ethanol extract can reduce the urate level in serum and protect renal function,the mechanism of lowering uric acid was not through the route of common intervening the activity of xanthine oxidase.

Key words: Pteridium aquilinum, ethanol extracts, hyperuricemia, uric acid, xanthine oxidase, renal

中图分类号: 

R285.5