化瘀解毒中药抑制梗阻性肾病巨噬细胞浸润减轻炎性损伤的作用

doi:10.16333/j.1001-6880.2019.2.005

天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (2): 211-215.doi: 10.16333/j.1001-6880.2019.2.005

化瘀解毒中药抑制梗阻性肾病巨噬细胞浸润减轻炎性损伤的作用

熊云昭1,2，王萱1,2，常奕2,3，王筝2,3，郝娟1,2，柴亚男1,2，王香婷2,3，许庆友2,3*

1河北中医学院研究生学院，石家庄 050091；2河北中医学院中西医结合学院，石家庄 050017；3河北省中西医结合肝肾病证重点实验室，石家庄 050091

出版日期:2019-03-07 发布日期:2019-03-07
基金资助:
国家自然科学基金（81473652）；河北省自然科学基金（H2015423009，H2016423059）；河北省高等学校科学技术研究项目（QN 2015144，ZD 20166018）

Inhibitory effects of Huayu Jiedu decoction on macrophage infiltration to alleviate infalmmatory injury in obstructive nephropathy rats

XIONG Yun-zhao1,2,WANG Xuan1,2,CHANG-Yi2,3,WANG Zheng2,3,HAO Juan1,2,CHAI Ya-nan1,2,WANG Xiang-ting2,3,XU Qing-you2,3*

1Graduate School,Hebei University of Chinese Medicine,Shijiazhuang 050091,China; 2College of Integrated Chinese and Western Medicine,Hebei University of Chinese Medicine,Shijiazhuang 050017,China; 3Hebei Key Laboratory of Integrative Medicine on Liver-kidney patterns,Shijiazhang 050091,China

Online:2019-03-07 Published:2019-03-07

摘要/Abstract

摘要： 为观察化瘀解毒中药对梗阻性肾病巨噬细胞浸润的影响及作用机制。将48只健康雄性Wistar大鼠随机分为假手术组、模型组、依普利酮组、中药组，每组12只。除假手术组外，其余大鼠结扎单侧输尿管（UUO）复制肾间质纤维化动物模型。治疗组分别给以依普利酮（100 mg/kg/d 加入饲料喂养）和化瘀解毒中药煎剂（14 g/kg/d 灌胃）。10 d后摘取肾脏，观察大鼠肾脏组织病理改变。免疫组化法标记巨噬细胞浸润，免疫组化、Western Blot方法检测血清和糖皮质激素诱导蛋白激酶1（SGK-1）、单核细胞趋化蛋白-1（MCP-1)、白细胞介素-1（IL-1）、肿瘤坏死因子-α（TNF-α）的表达。肾脏病理显示，UUO组大鼠肾脏有明显的肾小管扩张及上皮细胞脱落，间质巨噬细胞浸润增多和细胞外基质（ECM）大量积聚，SGK-1、MCP-1、IL-1、TNF-α表达明显增强。化瘀解毒中药可明显减轻UUO大鼠肾脏巨噬细胞等炎性细胞浸润和ECM沉积，下调SGK-1、MCP-1、IL-1、TNF-α表达。以上结果说明化瘀解毒中药可抑制梗阻性肾病诱导的巨噬细胞浸润，减轻肾脏炎性损伤。

关键词: 化瘀解毒中药, 醛固酮, 肾脏炎性损伤, 巨噬细胞, 单侧输尿管结扎

Abstract: To observe the effect of Huayu Jiedu decoction on macrophages infiltration in obstructive nephropathy rats,48 male Wistar rats were randomly divided into sham group,UUO group,EPL group and ZY group (n=12).Except the rats in sham group,the rats in the other groups were prepared unilateral ureteral obstruction (UUO) for renal interstitial fibrosis model.The rats were treated with 14 g/kg/d decoction of Chinese medicine in ZY group orally,and eplerenone at 100 mg/kg/d added to diet in EPL group.The kidneys were harvested to observe the histopathological changes at 10th day after surgery.Macrophage infiltration marked with F4/80 was examined by immunohistochemistry.The Serum and glucocorticoid induced kinase-1(SGK-1),Monocyte chemotactic protein-1(MCP-1),interleukin-1(IL-1) and tumor necrosis factor-α(TNF-α) were detected with immunohistochemistry and western blot.Renal pathology showed that renal tubular dilatation and epithelial cell shedding in UUO rats significantly.The infiltrated macrophage and accumulated extracellular matrix (ECM) also increased in interstitium of UUO rats markedly.The macrophages infiltration and ECM accumulation were attenuated by treated with Chinese herb decoction and eplereneone.Expressions of SGK-1,MCP-1,IL-1 and TNF-α were up-regulated in the UUO rats and were suppressed by Chinese herb decoction.Chinese herb decoction could inhibit the inflammatory injury via reducing macrophage infiltration in UUO rats.

Key words: Huayu Jiedu decoction, aldosterone, inflammatory injury of kidney, macrophage, UUO

中图分类号:

R285

熊云昭1,2，王萱1,2，常奕2,3，王筝2,3，郝娟1,2，柴亚男1,2，王香婷2,3，许庆友2,3*. 化瘀解毒中药抑制梗阻性肾病巨噬细胞浸润减轻炎性损伤的作用[J]. 天然产物研究与开发, 2019, 31(2): 211-215.

XIONG Yun-zhao1,2,WANG Xuan1,2,CHANG-Yi2,3,WANG Zheng2,3,HAO Juan1,2,CHAI Ya-nan1,2,WANG Xiang-ting2,3,XU Qing-you2,3*. Inhibitory effects of Huayu Jiedu decoction on macrophage infiltration to alleviate infalmmatory injury in obstructive nephropathy rats[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2019, 31(2): 211-215.

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化瘀解毒中药抑制梗阻性肾病巨噬细胞浸润减轻炎性损伤的作用

Inhibitory effects of Huayu Jiedu decoction on macrophage infiltration to alleviate infalmmatory injury in obstructive nephropathy rats

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