天然产物研究与开发 ›› 2018, Vol. 30 ›› Issue (4): 582-587.doi: 10.16333/j.1001-6880.2018.4.008

• 研究论文 • 上一篇    下一篇

黑茶提取物对脓毒症模型小鼠的保护作用

龙伟,牟感恩,张广慧,董辉,吴红英,周则卫,孙元明*   

  1. 中国医学科学院&北京协和医学院 放射医学研究所,天津 300192
  • 出版日期:2018-05-07 发布日期:2018-05-07
  • 基金资助:

    国家自然科学基金(81673106);中国医学科学院医学与健康科技创新工程(2017-I2M-1-012)

Dark Tea Extracts Protect Mice from Sepsis

LONG Wei, MU Gan-en, ZHANG Gang-hui, DONG Hui, WU Hong-ying, ZHOU Ze-wei, SUN Yuan-ming*   

  1. Institute of Radiation Medicine,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300192,China
  • Online:2018-05-07 Published:2018-05-07

摘要: 研究黑茶提取物对脓毒症模型小鼠的保护作用。本实验采用腹腔注射高浓度40 mg/kg脂多糖(LPS)和盲肠结扎穿孔术(CLP)两种不同的方式建立小鼠脓毒症模型,考察黑茶提取物对脓毒症小鼠7 d存活率的影响;利用LPS诱导巨噬细胞程序性坏死实验研究黑茶提取物对小鼠脓毒症治疗作用的机制。结果显示,黑茶水提物可提高LPS和CLP诱导的脓毒症小鼠的7 d存活率,提高幅度达40%;黑茶多糖可对LPS诱导的巨噬细胞程序性坏死有一定的抑制作用,与LPS对照组相比,差异具有显著性(P<0.05)。提示黑茶提取物对脓毒症模型小鼠有一定的治疗作用,其作用的机制可能与黑茶多糖抑制LPS诱导的巨噬细胞程序性坏死有关,更深入的作用机制有待进一步研究。

关键词: 黑茶, 脓毒症, 巨噬细胞

Abstract: This study aimed to investigate the dark tea extracts on the treatment of sepsis.Sepsis model mice were established by intraperitoneal injection of 40 mg/kg of lipopolysaccharide (LPS) and cecal ligation plus puncture(CLP),which were used to observe the 7 d survival of sepsis mice treated with dark tea extracts.Cell experiments were carried out to study the effect of dark tea extracts on the necroptosis of Macrophages induced by 10 μg/mL LPS,which was to further study the mechanism of treatment on sepsis.The results showed that dark tea water extract can improve the average survival time and 7 d survival rate of sepsis mice (40% lifted) induced by 40 mg/kg LPS and CLP;dark tea polysaccharide can inhibit the necroptosis of Macrophages induced by LPS (10 μg/mL),the difference was significant (P<0.05) when compared with LPS group.All these experimental results suggested that Dark tea extracts had a certain therapeutic effect on sepsis mice,which may be concerned with inhibition of macrophage necroptosis induced by LPS (10 μg/mL).Their mechanism need to be further study.

Key words: dark tea, sepsis, macrophage

中图分类号: 

R979.9