天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (12): 1998-2003.doi: 10.16333/j.1001-6880.2021.12.002

• 研究论文 • 上一篇    下一篇

橄榄苦苷对酪氨酸酶抑制作用的研究

陈静1，魏鉴腾2,3，裴栋2,3，陈文雅1，刘建飞2,3，邸多隆2,3，刘晔玮1*   

  1. 1兰州大学公共卫生学院营养与食品卫生学研究所；2中国科学院兰州化学物理研究所；3中科院西北特色植物资源化学重点实验室，兰州 730000

  • 出版日期:2021-12-28 发布日期:2021-12-29
  • 基金资助:
    国家重点研发计划（2019YFD1002404）；甘肃省科技重大专项（21ZD4NK045）

Study on the inhibitory effect of oleuropein on tyrosinase

CHEN Jing1,WEI Jian-teng2,3,PEI Dong2,3,CHEN Wen-ya1,LIU Jian-fei2,3,DI Duo-long2,3,LIU Ye-wei1*   

  1. 1Institute of Nutrition and Food Hygiene,School of Public Health,Lanzhou University;2Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences;3Key Laboratory of Chemistry of Northwestern Plant Resources,Chinese Academy of Sciences,Lanzhou 730000,China

  • Online:2021-12-28 Published:2021-12-29

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摘要：

本文通过测定橄榄苦苷对小鼠B16黑色素瘤细胞内酪氨酸酶和蘑菇酪氨酸酶的抑制率、分析橄榄苦苷对蘑菇酪氨酸酶的抑制机理和抑制类型，研究其对酪氨酸酶的抑制作用。结果表明，橄榄苦苷对小鼠B16黑色素瘤细胞酪氨酸酶的活性有明显的抑制作用，100、200 μmol/L的橄榄苦苷对B16细胞酪氨酸酶活性的抑制率分别为8.96%±2.53%和33.41%±3.74%。橄榄苦苷对蘑菇酪氨酸酶有一定的抑制活性，其IC50为0.210 mmol/L。动力学结果表明，橄榄苦苷对蘑菇酪氨酸酶的抑制作用类型为可逆性非竞争性抑制。综上，橄榄苦苷对B16细胞酪氨酸酶和蘑菇酪氨酸酶均有较强的抑制作用。本实验结果可为橄榄苦苷作为美白原料的开发提供一定的实验基础和理论依据。

关键词: 橄榄苦苷, 酪氨酸酶, 小鼠B16黑色素瘤细胞, 抑制活性, 动力学

Abstract:

In the paper,we determined the inhibition rate of oleuropein on mushroom tyrosinase and tyrosinase in mouse B16 melanoma cells,analyzed the inhibition mechanism and type of oleuropein on mushroom tyrosinase,in order to evaluate its inhibitory effect on tyrosinase.The results showed that oleuropein had a significant inhibitory effect on tyrosinase activity in mouse B16 melanoma cells,and the inhibition rates of 100 and 200 μmol/L oleuropein on tyrosinase activity in B16 cells were 8.96%±2.53% and 33.41%±3.74%,respectively.Oleuropein has a certain inhibitory activity on mushroom tyrosinase,and its IC50 is 0.210 mmol/L.The kinetic results showed that the inhibitory effect of oleuropein on mushroom tyrosinase was reversible non-competitive inhibition.In summary,oleuropein had a strong inhibitory effect on tyrosinase B16 cells and mushroom tyrosinase.The experimental results can provide a certain experimental and theoretical basis for the development of oleuropein as a whitening raw material.

Key words: oleuropein, tyrosinase, B16 melanoma cells, inhibitory activity, kinetics

中图分类号:  Q554.9

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