天然产物研究与开发 ›› 2022, Vol. 34 ›› Issue (11): 1930-1938.doi: 10.16333/j.1001-6880.2022.11.014

• 开发研究 • 上一篇    下一篇

解有机磷细菌对滇重楼根茎、须根及根际土壤重金属元素的影响

李卓蔚,袁   林,叶明燕,兰国新*,周   浓*   

  1. 重庆三峡学院 生物与食品工程学院,重庆 404120
  • 出版日期:2022-11-25 发布日期:2022-11-25
  • 基金资助:
    重庆市自然科学基金(cstc2018jcyjAX0267);重庆三峡学院研究生科研创新项目(YJSKY22028)

Effects of organophosphate-degradation bacteria on heavy metal elements in Paris polyphylla var. yunnanensis rhizomes, fibrous roots and rhizosphere soils

LI Zhuo-wei,YUAN Lin,YE Ming-yan,LAN Guo-xin*,ZHOU Nong*   

  1. College of Food and Biology Engineering,Chongqing Three Gorges University,Chongqing 404120,China
  • Online:2022-11-25 Published:2022-11-25

摘要:

探究接种不同解有机磷细菌后滇重楼根茎、须根及根际土壤中重金属残留量的变化规律,为滇重楼人工种植过程中重金属污染修复提供参考。通过室内盆栽接种试验,研究了在灭菌土壤接种不同解有机磷细菌对滇重楼根茎、须根及根际土壤中汞(Hg)、镉(Cd)、铅(Pb)、铬(Cr)、砷(As)5种重金属残留量的影响。与对照组(CK组)相比,接种不同解有机磷细菌对滇重楼根茎及须根中5种重金属残留量影响各不相同,对重金属Pb的修复效果最明显,其中三种菌(Bacillus mycoidesB. wiedmanniiB. proteolyticus)混合处理组(S7组)对滇重楼重金属的修复效果最佳。此外,单项污染指数和内梅罗污染指数表明,除B.mycoides处理组的土壤存在污染风险以外,其余处理组均达到清洁水平。同时,5种重金属元素在滇重楼根茎和须根中的富集能力各不相同,富集系数最大为Cd,最小为Cr,S7组滇重楼根茎中的富集能力相对较小。相关性分析表明,滇重楼根茎和须根中的Hg和Pb元素呈显著正相关(P<0.05),相关系数分别为0.761、0.811。综上,接种解有机磷细菌能够有效降低滇重楼根茎及须根中Hg、Pb和Cr元素的含量,其中S7组接种处理的效果最佳。

关键词: 滇重楼, 解有机磷细菌, 重金属, 富集系数

Abstract:

To explore the change rule of heavy metal residues in rhizomes,fibrous roots and rhizosphere soils of Paris polyphylla var. yunnanensis inoculated with different organophosphate-degradation bacteria (OPDB),which provides a reference for heavy metal pollution remediation during artificial planting of Paris polyphylla var. yunnanensis.Through indoor pot plant inoculation experiments,the effects of residues of inoculating different OPDB on mercury (Hg),cadmium (Cd),lead (Pb),chromium (Cr),and arsenic (As) heavy metals in rhizomes,fibrous roots and rhizosphere soils of  Paris polyphylla var. yunnanensis were studies.Compared with the control group,inoculation with different OPDB had different differences in the residues of five heavy metals in rhizomes and fibrous roots of Paris polyphylla var. yunnanensis,and the restoration effect on the heavy metal Pb was the most obvious.The S7 group (Bacillus mycoides,B. wiedmannii,B. proteolyticus) has the most obvious repair effect on heavy metals.In addition,the Single pollution index and the Nemerow pollution index showed that,except for the soil in the B. mycoides treatment group,which had a risk of contamination,the rest of the treatment groups reached a clean level.At the same time,the enrichment abilities of the five heavy metal elements in rhizomes and fibrous roots of Paris polyphylla var. yunnanensis were different,with the maximum enrichment coefficient was Cd,and the minimum was Cr.The enrichment capacity in the rhizomes of Paris polyphylla var. yunnanensis in S7 group was relatively small.The correlation analysis showed that Hg and Pb elements in rhizomes and fibrous roots of Paris polyphylla var. yunnanensis were significantly positively correlated (P<0.05),and the correlation coefficients were 0.761 and 0.811,respectively.It can be seen that the content of Hg,Pb and Cr in the rhizomes and fibrous roots of Paris polyphylla var. yunnanensis can be effectively reduced by inoculation with OPDB,and the S7 group has the best effect.

Key words: Paris polyphylla , var. , yunnanensis, organophosphate-degradation bacteria, heavy metal, enrichment coefficients

中图分类号:  R282.2