天然产物研究与开发 ›› 2025, Vol. 37 ›› Issue (7): 1322-1330.doi: 10.16333/j.1001-6880.2025.7.014 cstr: 32307.14.1001-6880.2025.7.014

• 开发研究 • 上一篇    下一篇

微波辅助纤维素酶提取辣木籽多酚工艺优化及其活性研究

茹昕昕1，赵一阳2，张万忠3*，朱建星2*   

  1. 1沈阳化工大学化学工程学院；2沈阳化工大学环境与安全工程学院；3沈阳化工大学图书馆，沈阳 110142
  • 出版日期:2025-07-28 发布日期:2025-07-28

Optimization of microwave-assisted cellulase extraction of polyphenols from Moringa oleifera seeds and its activity

RU Xin-xin1,ZHAO Yi-yang2,ZHANG Wan-zhong3*,ZHU Jian-xing2*   

  1. 1School of Chemical Engineering,Shenyang University of Chemical Technology; 2School of Environmental and Safety Engineering,Shenyang University of Chemical Technology;3Shenyang University of Chemical Technology Library,Shenyang 110142,China
  • Online:2025-07-28 Published:2025-07-28

PDF (PC)

6

摘要：

为优化辣木籽多酚提取工艺，采用微波辅助纤维素酶法进行提取，研究酶添加量、微波时间、酶解时间、乙醇体积分数对辣木籽多酚提取量的影响，运用响应面法优化提取工艺。结果表明最佳条件为：酶添加量0.4%、乙醇体积分数55%、酶解时间35 min、微波时间7 min，提取量为19.23 mg/g，与预测值无显著性差异，是传统方法提取量的3到5倍。当辣木籽多酚浓度为10.24 mg/mL时，对ABTS与DPPH自由基清除率为91.4%与90.7%，清除效率分别达到了同浓度下VC的91.6%与90.8%，对α-淀粉酶的抑制率达66.8%，表明了辣木籽多酚提取物具有较好的抗氧化活性及淀粉酶抑制能力。

关键词: 辣木籽, 微波辅助纤维素酶法, 多酚, 响应面, 抗氧化活性, 淀粉酶抑制能力

Abstract:

To optimize the extraction process of polyphenols from Moringa oleifera seeds,microwave-assisted cellulase method was used for extraction.The effects of enzyme addition amount,microwave time,enzymatic hydrolysis time,and ethanol volume fraction on the extraction amount of polyphenols from M.oleifera seeds were studied,and response surface methodology was used to optimize the extraction process.The results showed that the optimal conditions were:enzyme addition of 0.4%,ethanol volume fraction of 55%,enzymatic hydrolysis time of 35 min,microwave time of 7 min.The extraction amount was 19.23 mg/g,which was not significantly different from the predicted value and was 3 to 5 times higher than the extraction amount obtained by traditional methods.When the concentration of polyphenols from M.oleifera seeds was 10.24 mg/mL,the clearance rates of ABTS and DPPH free radicals reached 91.4% and 90.7%,at the same concentration,the clearance efficiency respectively reached 91.6% and 90.8% of VC,and the inhibition rate of α-amylase reached 66.8%,indicating that polyphenol from M.oleifera seeds extract has good antioxidant activity and inhibitory ability on amylase.

Key words: Moringa oleifera seeds, microwave assisted cellulase method, polyphenols, response surface, antioxidant activity, amylase inhibition ability

中图分类号:  TS201.1

版权所有 © 《天然产物研究与开发》编辑部
地址：四川天府新区群贤南街289号　邮编：610299
电话：028-85210304　QQ群：342035423　Email：trcw@clas.ac.cn　
本系统由北京玛格泰克科技发展有限公司设计开发
总访问量：　今日访问：　在线人数：