天然产物研究与开发 ›› 2025, Vol. 37 ›› Issue (7): 1331-1340.doi: 10.16333/j.1001-6880.2025.7.015 cstr: 32307.14.1001-6880.2025.7.015

• 开发研究 • 上一篇    下一篇

响应面法优化超声辅助提取沙棘叶多酚工艺及其抗氧化和降脂活性研究

肖付才*，邢晓轲，李颖奎，黄斐   

  1. 许昌职业技术学院园林与食品工程学院，许昌 461000

  • 出版日期:2025-07-28 发布日期:2025-07-28
  • 基金资助:
    河南省教育厅质量工程项目(2022-6)

Optimization of ultrasound-assisted extraction of polyphenols from Hippophae rhamnoides leaf by response surface methodology and its antioxidant and lipid-lowering activities

XIAO Fu-cai*,XING Xiao-ke,LI Ying-kui,HUANG Fei   

  1. College of Landscape and Food Engineering,Xuchang Vocational Technical College,Xuchang 461000,China
  • Online:2025-07-28 Published:2025-07-28

PDF (PC)

3

摘要：

优化沙棘叶多酚（Hippophae rhamnoides leaf polyphenols，HRLP）的超声辅助提取工艺，并评价其抗氧化与降脂活性。研究超声时间、料液比、超声功率和超声温度4个因素对HRLP提取量的影响，在单因素试验的基础上采用Box-Behnken响应面法优化提取工艺参数，最优提取条件得到的HRLP进行体外实验来验证其抗氧化和降脂活性。结果表明：在超声时间42 min、温度61 ℃、功率410 W、料液比1∶47（g/mL）条件下多酚提取量最高，可达到18.07 ± 0.74 mg/g。在此条件下得到的HRLP对ABTS、DPPH以及羟基自由基清除能力IC50值分别为0.11、0.25、0.54 mg/mL，对铁离子还原能力约为维生素C的1/6。降脂活性研究发现，HRLP最大油脂吸附量为38.7 mg/g，胆汁酸吸附率为62.45%，胰脂肪酶抑制活性IC50为0.85 mg/mL。HRLP提取工艺稳定可行，所得HRLP具有良好的抗氧化与降脂活性。该研究所建立的新方法操作简便易行，可实现HRLP的高效制备，为HRLP的工业化生产提供了新思路和新方法。

关键词: 沙棘叶, 多酚, 超声提取, 响应面优化, 抗氧化活性, 降脂活性

Abstract:

This study aimed to optimize the ultrasound-assisted extraction process of Hippophae rhamnoides leaf polyphenols (HRLP) and evaluate their antioxidant and lipid-lowering activities. The effects of four factors—ultrasonic time, solid-to-liquid ratio, ultrasonic power, and temperature—on HRLP yield were investigated. Based on single-factor experiments, the extraction parameters were optimized using the Box-Behnken response surface methodology. The HRLP obtained under optimal conditions was further subjected to in vitro experiments to validate its antioxidant and lipid-lowering activities. Results showed that the maximum polyphenol yield of 18.07 ± 0.74 mg/g was achieved under the optimal conditions: ultrasonic time of 42 min, temperature of 61 °C, power of 410 W, and solid-to-liquid ratio of 1∶47 (g/mL). The HRLP exhibited IC50 values of 0.11, 0.25, and 0.54 mg/mL for scavenging ABTS, DPPH, and hydroxyl radicals, respectively, and its ferric ion reducing capacity was approximately 1/6 that of vitamin C. In lipid-lowering studies, HRLP demonstrated a maximum oil-binding capacity of 38.7 mg/g, a bile acid binding rate of 62.45%, and pancreatic lipase inhibitory activity with an IC50 of 0.85 mg/mL. The extraction process proved stable and feasible, yielding HRLP with significant antioxidant and lipid-lowering properties. The novel method established in this study features simple operation and enables efficient preparation of HRLP, providing innovative strategies for its industrial-scale production.

Key words: Hippophae rhamnoides leaf, polyphenols; ultrasound-assisted extraction, response surface optimization, antioxidant activity, lipid-lowering activity

中图分类号:  R285.5 TS201.4 

版权所有 © 《天然产物研究与开发》编辑部
地址：四川天府新区群贤南街289号　邮编：610299
电话：028-85210304　QQ群：342035423　Email：trcw@clas.ac.cn　
本系统由北京玛格泰克科技发展有限公司设计开发
总访问量：　今日访问：　在线人数：