天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (1): 114-117.doi: A

• 开发研究 • 上一篇    下一篇

响应面设计法优化仙鹤草总多酚的超声提取工艺

徐秀泉1*,李 峰2,汤 建1,许 源1   

  1. 1江苏大学药学院,镇江 212013;2江苏大学附属医院,镇江 212001
  • 收稿日期:2011-03-18 出版日期:2012-01-30 发布日期:2012-02-15
  • 基金资助:

    镇江市社会发展项目(SH2010033)

Optimization of Ultrasonic-assisted Extraction of Total Polyphenols from Agrimonia pilosa Ledeb.by Response Surface Methodology

XU Xiu-quan1*,LI Feng2,TANG Jian1,XU Yuan1   

  1. 1College of Pharmacy,Jiangsu University,Zhenjiang 212013,China,2Affiliated Hospital of Jiangsu University,Zhenjiang 212001,China
  • Received:2011-03-18 Online:2012-01-30 Published:2012-02-15

摘要: 在单因素实验的基础上,采用响应面分析法对影响超声辅助提取仙鹤草总多酚得率的主要因素(超声温度、料液比和乙醇浓度)进行优化,建立了影响因素与总多酚之间的函数关系。获得最佳工艺条件为:71%乙醇,料液比1:24,超声提取温度60 ℃,提取时间20 min。在此最佳条件下,总多酚得率为3.56%,试验结果与模型预测值相符。

关键词: 仙鹤草, 总多酚, 超声提取, 响应面设计法

Abstract: Response surface methodology (RSM) was used to optimize ultrasonic-assisted extraction for total polyphenols from Agrimonia pilosa Ledeb.based on single-factor experiment.The main factors extraction temperature,liquid-material ratio and ethanol concentration were determined.The relationship between influencing factors and response values was established.The optimum extraction conditions were as follows: the extracting solvent was 71% ethanol,solid-liquid ratio was 1:24,extracting temperature was 60℃,and extracting time was 20 min.Under these conditions,the maximal yield of total polyphenols reached 3.56%,which was well matched with the predicted concent.

Key words: Agrimonia pilosa Ledeb., total polyphenols, ultrasonic-assisted extraction, response surface methodology

中图分类号: 

R284.2