基于响应面法从连翘叶中提取连翘酯苷A和连翘苷的工艺优化

doi:A

摘要/Abstract

摘要： 采用响应面分析法优化连翘叶中连翘酯苷A和连翘苷的最佳提取工艺条件，在单因素实验基础上，采用 Box-Behnken 中心组合设计原理研究液料比、乙醇浓度、浸提时间、浸提温度4个因素对连翘酯苷A得率和连翘苷得率的影响。利用Design Expert 8.0.5 b分析确定最佳提取工艺，根据实际条件，得到连翘酯苷A和连翘苷的最佳提取工艺为：乙醇浓度 53%、提取温度74 ℃、浸提时间为53 min、液料比30 mL/g。回归模型预测最优条件下连翘酯苷A得率为7.53%，连翘苷得率为3.03%，验证值分别为7.56%，3.09%，与预测值的相对误差分别为0.4%，2.0%。

关键词: 连翘叶, 连翘酯苷A, 连翘苷, 响应面法, HPLC

Abstract: Response surface methodology (RSM) was applied to optimize the extraction technology of forsythiaside and phillyrin from forsythia suspense leaves. Base on single factor experiment, the influence factors of liquid to solid ration, ethanol concentration, extraction time and extraction temperature were studied on the yields of forsythiaside and phillyrin. According to Box-Behnken combination method (Design expert 8.0.5 b), the optimal conditions were determined as follows: ratio of liquid to solid 30 mL/g, ethanol concentration 53%, extraction time 53 min, and extraction temperature 74 ℃. Under the conditions, the yields of forsythiaside and phillyrin were 7.56% and 3.09%, respectively, and the relative error between the testing values (7.53% and 3.03%, respectively) and theoretic values were 0.4% and 2.0%, respectively.

Key words: forsythia suspense leaves, response surface methodology, forsythiaside, phillyrin, HPLC

中图分类号: R931.71

刘伟霞，杨建雄. 基于响应面法从连翘叶中提取连翘酯苷A和连翘苷的工艺优化[J]. 天然产物研究与开发, 2012, 23(8): 1098-.

LIU Wei-xia, YANG jian-xiong. Optimization of Extraction Technology of Forsythiaside and Phillyrin in Forsythia suspense Leaves Based on Response Surface Methodology[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(8): 1098-.

参考文献

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