藏红花花瓣和雄蕊挥发油化学成分GC-MS分析及比较

doi:A

天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (09): 1239-.doi: A

藏红花花瓣和雄蕊挥发油化学成分GC-MS分析及比较

王晓萌¹，叶扬¹，白洁^1*，郑建佳²，官兆龙²，鲁晓莉²，陈放¹

采用水蒸气蒸馏法从藏红花花瓣和雄蕊中提取挥发油，用GC-MS技术结合计算机检索对其二者化学成分进行分离和鉴定，用色谱峰面积归一化法计算各组分的相对含量。花瓣中共鉴定出16种化合物，主要成分为正二十六烷（11.60%）、正十五烷（11.31%）、棕榈酸甲酯（10.82%）、油酸甲酯（10.35%）、2，4-二叔丁基苯酚（9.63%）、亚油酸甲酯（7.18%）、藏红花醛（5.66%）；雄蕊中共鉴定出20种化合物，主要成分为油酸甲酯（30.83%）、亚油酸甲酯（24.12%）、环己醇（16.80%）、硬脂酸甲酯（12.88%）、棕榈酸甲酯（8.97%）、花生酸甲酯（1.18%）、苯并噻唑（1.01%）。

出版日期:2012-09-29 发布日期:2013-01-06
基金资助:
国家科技支撑计划：中药有效成分群辨识技术研究（2008BAI51B01）；西红花规模化生产技术服务

Analysis and Comparison of the Volatile Oils from Crocus sativus by GC-MS

WANG Xiao-meng¹, YE Yang¹, BAI Jie¹, ZHENG Jian-jia², GUAN Zhao-long², LU Xiao-li², CHEN Fang¹

The volatile oils were extracted from petals and stamens of Crocus sativus by steam distillation. The chemical compounds were separated and identified by capillary GC-MS method. The relative content of each component was calculated by area normalization. Sixteen compounds were identified from petals of C. sativus. The main components were n-hexacosane (11.60%), n-pentadecane (11.31%), palmitic acid methyl ester (10.82%), oleic acid methyl ester (10.35%), 2,4-ditertiary butyl phenol (9.63%), linoleic acid methyl ester (7.18%) and safranal (5.66%). Twenty compounds were identified from stamens of C. sativus. The main components were oleic acid methyl ester (30.83%), linoleic acid methyl ester (24.12%), cyclohexanol (16.80%), stearic acid methyl ester (12.88%), palmitic acid methyl ester (8.97%), eicosanoic acid methyl ester (1.18%) and benzothiazole (1.01%).

Online:2012-09-29 Published:2013-01-06

摘要/Abstract

摘要： 采用水蒸气蒸馏法从藏红花花瓣和雄蕊中提取挥发油，用GC-MS技术结合计算机检索对其二者化学成分进行分离和鉴定，用色谱峰面积归一化法计算各组分的相对含量。花瓣中共鉴定出16种化合物，主要成分为正二十六烷（11.60%）、正十五烷（11.31%）、棕榈酸甲酯（10.82%）、油酸甲酯（10.35%）、2，4-二叔丁基苯酚（9.63%）、亚油酸甲酯（7.18%）、藏红花醛（5.66%）；雄蕊中共鉴定出20种化合物，主要成分为油酸甲酯（30.83%）、亚油酸甲酯（24.12%）、环己醇（16.80%）、硬脂酸甲酯（12.88%）、棕榈酸甲酯（8.97%）、花生酸甲酯（1.18%）、苯并噻唑（1.01%）。

关键词: 藏红花, 水蒸气蒸馏, 挥发油, GC-MS

Abstract: The volatile oils were extracted from petals and stamens of Crocus sativus by steam distillation. The chemical compounds were separated and identified by capillary GC-MS method. The relative content of each component was calculated by area normalization. Sixteen compounds were identified from petals of C. sativus. The main components were n-hexacosane (11.60%), n-pentadecane (11.31%), palmitic acid methyl ester (10.82%), oleic acid methyl ester (10.35%), 2,4-ditertiary butyl phenol (9.63%), linoleic acid methyl ester (7.18%) and safranal (5.66%). Twenty compounds were identified from stamens of C. sativus. The main components were oleic acid methyl ester (30.83%), linoleic acid methyl ester (24.12%), cyclohexanol (16.80%), stearic acid methyl ester (12.88%), palmitic acid methyl ester (8.97%), eicosanoic acid methyl ester (1.18%) and benzothiazole (1.01%).

Key words: Crocus sativus, steam distillation, volatile oil, GC-MS

中图分类号:

Q946.91　

王晓萌，叶扬，白洁，郑建佳，官兆龙，鲁晓莉，陈放. 藏红花花瓣和雄蕊挥发油化学成分GC-MS分析及比较[J]. 天然产物研究与开发, 2012, 23(09): 1239-.

WANG Xiao-meng, YE Yang, BAI Jie, ZHENG Jian-jia, GUAN Zhao-long, LU Xiao-li, CHEN Fang. Analysis and Comparison of the Volatile Oils from Crocus sativus by GC-MS[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(09): 1239-.

参考文献

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藏红花花瓣和雄蕊挥发油化学成分GC-MS分析及比较

Analysis and Comparison of the Volatile Oils from Crocus sativus by GC-MS

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