天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (3): 453-461.doi: 10.16333/j.1001-6880.2021.3.013

• 开发研究 • 上一篇    下一篇

乌鳖黑色素理化性质及其抗氧化活性研究

项锦敏,赵琼瑜,远航,彭振辉,宋伟*   

  1. 浙江万里学院生物与环境学院,宁波 315100
  • 出版日期:2021-03-28 发布日期:2021-04-01
  • 基金资助:
    新品种选育重大科技专项(2016C0 2055-4-1);宁波市重大科技专项(2017C110012);浙江省教育厅一般科研项目(Y201738596)

Physicochemical properties and antioxidant activities of melanin from Chinese soft-shelled turtle (Pelodiscus sinensis)

XIANG Jin-min,ZHAO Qiong-yu,YUAN Hang,PENG Zhen-hu,SONG Wei*   

  1. College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,China
  • Online:2021-03-28 Published:2021-04-01

摘要:

本试验以乌鳖肌肉为原料,采用“脱脂-酶解-酸解”三步法制备乌鳖黑色素,研究其结构特性、理化性质和抗氧化活性。采用紫外分光光度计、傅里叶红外光谱仪和元素分析仪对乌鳖肌肉黑色素结构特性进行分析,并进行了颜色检测、溶解性试验、稳定性试验、总抗氧化能力试验、羟基自由基清除能力试验、DPPH自由基清除能力试验、超氧阴离子自由基清除能力试验等理化性质和抗氧化活性检测分析。结果表明:乌鳖黑色素粉末呈黑色(L*=45.04),略带红色和黄色(a* = 1.92,b* = 5.27);具有与其他黑色素相似的溶解性,溶于碱性溶液,不溶于水、有机溶剂和酸性溶液等;乌鳖黑色素在1 h内具有较强光稳定性和热稳定性;乌鳖黑色素具有较强的抗氧化能力,其中2.0 mg/mL浓度的黑色素溶液羟自由基清除率、超氧阴离子自由基清除率和DPPH自由基清除率均在40%以上。

关键词: 乌鳖, 黑色素, 结构特性, 理化性质, 抗氧化活性

Abstract:

This study aimed to develop a method for extracting melanin in muscle of Chinese soft-shelled turtle by Skim-enzymatic hydrolysis and acid hydrolysis,and to study its structural characteristics,physicochemical property,as well as antioxidant activity.The melanin was examined by ultraviolet-visible spectroscopy,fourier transform infrared spectroscopy and elemental analyzer,before being evaluated by color detection,solubility test,stability test,hydroxyl radical assay,DPPH radical-scavenging assay and superoxide-radical scavenging assay.The result showed that the melanin powder was dark mixed with a little red and yellow (L* = 41.03,a* = 2.26,b* = 3.78).The solubility properties were similar to those of typical melanin.The melanin possessed a stronger thermal stability and light stability within 1 hour,as well as exhibited a strong antioxidant activity.At concentration of 2.0 mg/mL,the total antioxidant activity,hydroxyl radical scavenging activity,DPPH radical scavenging activity,and superoxide-radical scavenging activity of melanin were reduced more than 40%.

Key words: Pelodiscus sinensis, melanin, structural characteristics, physicochemical property, antioxidant activity

中图分类号:  TS254.1