NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2022, Vol. 34 ›› Issue (5): 800-809.doi: 10.16333/j.1001-6880.2022.5.008

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Paclitaxel content in mycorrhizal of Taxus chinensis var. mairei and isolation purification and species identification of its fungal 

PENG Feng-zhen1,DU Ya-tian1 *,ZHOU Chun-chang2,GONG Xue-yuan3   

  1. 1Key Laboratory of Hunan Forest Products and Chemical Industry Engineering,Jishou University,Zhangjiajie 427000,China;2School of Basic Medicine Sciences Zhejiang University,Hangzhou 310000,China;3Getein Biotech Incorporated Company,Nanjing 210044,China
  • Online:2022-05-28 Published:2022-05-23

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Abstract:

In this article,HPLC,tissue culture,morphological observation and biological analysis of fungal ribosomal transcription spacer (rDNA-ITS) sequence were used to research on the paclitaxel content in the mycorrhizal tissue aratomy each parts,stem callus of Taxus chinensis var. mairei and the stem callus after interaction with mycorrhizal fungi,and the isolation,purification and species identification of mycorrhizal fungi group.The results showed that the paclitaxel content in the mycorrhizal primordial root tissue of T. chinensis var. mairei was 0.645 mg/g higher than that of fibrous root,which was one order of magnitude higher than that of other organ tissues,and the interaction between stem callus and mycorrhizal fungi of T. chinensis var. mairei  could increase the content of paclitaxel.26 strains of mycorrhizal fungi that were isolated and purified from the mycorrhizal,belonging to three phyla,six classes,nine orders, 11 families and 14 genera, including  Aspergillus, Trichoderma, Fusarium, Penicillium, Cylindrodendrum, Cylindrocladiella, Clonostachys,Xylaria,Cladosporium,Corynespora,Paraboeremia,Pezicula, Colletotrichum and Bjerkandera.The first two genera are the dominant genera.The results showed that there were species diversity of the mycorrhizal fungi in the mycorrhizal of T. chinensis var. mairei ,and it was found that there were great differences between the species diversity of the mycorrhizal fungi in the mycorrhizal of T. chinensis var. mairei  and the species diversity of the endophytic fungi in the other organs and tissues as its roots,bark,phloem,wood and leaves,and indicating that the symbiotic interaction between mycorrhizal fungi and non corked roots of T. chinensis var. mairei  is the biological basis for its high paclitaxel accumulation.This paper provides a basis for the research on the correlation between its higher paclitaxel content and mycorrhizal fungi.

Key words: Taxus chinensis , var. , mairei , , plant endophytes, mycorrhiza, mycorrhizal fungus, strain isolation, species identification

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