The blood-brain barrier (BBB) is a key physiological defense of the brain,which controls the transport process between the blood and the brain,effectively blocks exogenous foreign bodies,and protects the central nervous system from damage.Meanwhile,the presence of BBB also makes it difficult for exogenous drugs such as active substances derived from traditional Chinese medicine to penetrate into the brain,which hinders the treatment of the CNS diseases.Therefore,accurate and simple animal BBB evaluation models are essential for the development of drugs for the treatment of encephalopathy.In this paper,a systematic review was summarized about the structure and function of the BBB of model animal zebrafish and the progress of active substances of traditional Chinese medicine in the penetration of BBB,in order to provide a new research method for the exploration of traditional Chinese medicine in the treatment of CNS diseases.