Abstract: The anthocyanin in the blackberry pomaces was extracted with the different metheods, and the results showed that the anthocyanin content of rasound-assisted extraction was the highest. The 60% ethanol was used to extract anthocyanin with ultrasonic assisted extraction and ratio of liquid to materia, ultrasoni time, extraction temperature, ultrasonic power, and extraction times were assessed in single-factors experiment. On the basis of single-factor test, the optimum conditions for the ultrasound assisted extraction of anthocyanin from blackberry pomaces were determined as follows using response surface method (RSM): the liquid-olid atio (mL:g) 14:1, extraction time 40 min, extraction temperature 58 ℃, ultrasonic wave power 300 W, and two step extraction. The correlation analysis results showed that the anthocyanin content and DPPH• scavenging activity had significant correlation, and inferenced that anthocyanin was the main free radical scavenging substances.

Key words: blackberry pomaces, anthocyanin, extraction, response surface methodology, radical