Methods for Depurating Urine of Rat by HPLC-DAD after Intragastric Administration with Bazhen Decoction

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Abstract

Abstract: To dispose the endogenous substance in urine and enrich the chemical constituent from Bazhen decoction, the urine sample of rat after intragastric administration with Bazhen decoction was depurated by many methods. To obtain the best methods, blank urine and drug-containing urine samples were extracted with n-butyl alcohol, ethyl acetate and SPE, respectively, and then were analyzed by HPLC. After analyzing the liquid chromatograms of urine samples, SPE extraction was determined as the best depurated methods, and the optimal gathering time for urine samples was 4-8 h.

Key words: Bazhen decoction, HPLC, urine, SPE, depurating methods

CLC Number:

R285

LI Wen-lan, YANG Yang, BAI Jing, DAI Qi-chang, JI Yu-bin. Methods for Depurating Urine of Rat by HPLC-DAD after Intragastric Administration with Bazhen Decoction[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(3): 325-328.

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Methods for Depurating Urine of Rat by HPLC-DAD after Intragastric Administration with Bazhen Decoction

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