HPLC Fingerprint of Dendrobium candidum from Guangxi

doi:A

Abstract

Abstract: The fingerprint of Dendrobium candidum from Guangxi was established by HPLC and quality of it was evaluated with the similarity. The results showed that similarity of 11 batches of D. candidum was higher than 0.8, and similarity of 2 batches was below 0.8 in the fingerprint with 13 common peaks,which demonstrated that there was a certain relationship between similarity and region of D. candidum. Peak area of the fingerprint of different parts from cultivated D. candidum was compared, which showed the medicinal materials should not be harvested in advance. In addition, similarity of 3 batches of tissue-cultured D. candidum was low, which indicated that the chemical composition of tissue cultures was of large difference at different periods.

Key words: Dendrobium candidum, HPLC, fingerprint

CLC Number:

R282
R917

HE Tie-guang, LU Sheng-xian, WANG Ai-qin, WEI Zhu-yang, HUANG Dao-ping, ZHOU Lin. HPLC Fingerprint of Dendrobium candidum from Guangxi[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(3): 353-358.

References

1 Wu FF(伍芬芳),Zeng L(曾玲).Briefly discussed Dendrobium candidum.Zhejiang J Integr Tradit Chin Western Med (浙江中西医结合杂志), 2004, 14(3): 184.
2 Wu HS (吴昊姝),Xu JH(徐建华),Chen LZ(陈立钻),et al.Studies on anti-hyperglycemic effect and its mechanism of Dendrobium candidum.Chin J Chin Mater Med (中国中药杂志),2004,29(2):160-163.
3 HE TG(何铁光),Yang LT(杨丽涛),LI YR(李杨瑞),et al.Effects of the polysaccharides DCPP1a-1 from suspension-cultured protocorms of Dendrobium candidum on oxygen radical and lipid peroxidation.Nat Prod Res Dev (天然产物研究与开发),2007,19(3):410-414.
4 Fan YJ,He XJ,Zhou SD,et al.Composition analysis and antioxidant activity of polysaccharide from Dendrobium denneanum. Int J Biol Macromol,2009,45(2):169-173.
5 Fu KC (付开聪),Feng DQ (冯德强),Zhang SY(张绍云),et al.Studies on high production technology in scale cultivation of Dendrobium officinale.Chin Tradit Herb Drug (中草药),2003,34 (2):177-179.
6 Bai MF(白美发),Huang M(黄敏).Study on high-efficiently and protected cultivation for Dendrobium candidum. J Anhui Agric Sci (安徽农业科学),2008, 36(35):15416, 15421.
7 Huang MQ (黄民权),Lu YJ(卢应京).Prospect on the callus culture of Dentrobium Candidum used as drugs.J Chin Med Mater (中药材),1998,21(11):543-545.
8 Lu SX(卢声仙),He TG(何铁光),Wang AQ(王爱勤),et al.Study on extraction method of chemical components of Dendrobium candidum. Southwest Chin J Agric Sci (西南农业学报),2010,23 (2):523-527.
9 State Drug Administration (国家药品监督管理局).Technical requirements of the fingerprint in injection of chinese materia medica (Tentative Standard). Chin Tradit Patent Med (中成药),2000,22 (10):671-675.
10 Zhang TB(张同波),Zhang HQ(张红芹).Similarity analysis of HPLC fingerprinting of Scutellaria barbata D.Don. Chin Healthcare Front (中国医疗前沿),2008,3 (22): 4-5.

[1]	DU Zhong-hua, XU Lei, HU Zhi-mei, ZHANG Ming, XIA Heng-jian, WANG Sheng-jun, YE Li-chun, SHI Zhao-hua. Quantitative value transfer of key substances at different stages of the preparation process of Prunellae Spica ointment [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(2): 368-383.
[2]	PENG Zhi-cheng, YANG Jie, LIANG Yue-yi, HOU Xu-xuan, LI Zhen-yu, LYU Wei-sheng, CHEN Rou-shan. Study on the quality marker of Lycii Cortex in the treatment of "three hypers" based on fingerprint,network pharmacology and quantitative analysis [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(2): 425-434.
[3]	TANG Lin, LU Ai-juan, HU Chao, LI Hai-ying, GAO Yuan-hang, LI Hao, YANG Lei. Spectrum-effect relationship between HPLC fingerprint and in vitro antioxidant activity of Notoginseng Radix et Rhizoma and its processed products [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(1): 1-12.
[4]	MA Sheng-ming, LI Miao-miao, HUANG Ming-feng, LIAN Kai-qi, WENG Shao-ting, HAO Jie, ZHANG Kun-peng. Effect of Ranunculus ternatus Thunb.in the treatment of ulcerative colitis based on UHPLC-MS/MS and network pharmacology [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(增刊1): 40-46.
[5]	LI Yong-dong, HUO Qin-jian, YANG Ming-hua, LI Yun-qiang, JIN Hui, YANG Min-min, LIAN Yun, LIU Jin-liang. Study on browning marker of tobacco leaf based on HPLC fingerprint and antioxidant spectrum-effect relationship [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(增刊1): 99-106.
[6]	ZENG Zi-hang, PEI Chao-yang, WU Meng-mei, CAO Hui, ZHANG Ying, WU Meng-hua, MA Zhi-guo. Comparative study on HPLC fingerprints and four constituents content of Atractylodis Macrocephalae Rhizoma before and after processing with rice-washed water [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(9): 1595-1604.
[7]	SUN Ying-di, ZHONG Ai-yu, ZHANG Chen-yang, ZHAO Li-yan, ZHANG Wan-ming, ZHANG Yuan-yuan. Establishment of UPLC fingerprint of Sophorae Flavescentis Radix and its anti-tumor spectral effect relationship [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(8): 1460-1470.
[8]	GUO Yan-hong, WU Min, LING Yun-zhi, ZHAO Hai, CHEN Zhi-yuan. Quality evaluation of Epilobium hirsutum based on multi-index component quantitative combined with chemometrics,weighted TOPSIS and grey relational analysis fusion model [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(7): 1279-1288.
[9]	DENG Cong-you, WANG Min-yu, XU Wen-hui, QIU Jia-jun, LIU Xuan-lin, CHEN Xiang-dong, HE Min-you. Quality evaluation of fried Viticis Fructus standard decoction based on fingerprint,chemometrics and multi-component content determination [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(6): 1104-1113.
[10]	ZHAN Zhi-hong, WEI Jia-bao, TANG Shuang-yan, XIE Jia-hui, HU Yu, ZHANG Li-jun. Component variation patterns and quality evaluation of Polygonati Rhizoma during wine processing based on HPLC fingerprint and multi-component dynamic analysis [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(5): 849-858.
[11]	GONG Wen-hui, GAO Lei, XIN Li, LIU Ming-gui, ZHANG Wen, JIN Shu-han, CHEN Xiao-yan, ZHANG Jin-lian. Quality evaluation of Hedysari Radix and its different processed products based on fingerprint and multi-component content determination combined with weighted TOPSIS [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(5): 930-943.
[12]	HUANG Chun-yan, HE Min, HE Qiu-yun, LIU Xian-fu, MO Yuan-yao, ZHOU Yi, MO Yong-fu, CHEN Ru-xu. Quality evaluation of Fici Hirtae Radix from different regions in Guangxi based on HPLC fingerprint and UPLC-MS/MS multi-index component content determination [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(4): 678-686.
[13]	WANG Chang, LIU Xiao-ya, ZHANG Peng, WANG Ya-ping, ZHAO Da-qing, LIU Li, QI Bin. Effect of various drying methods on the quality of fresh-cut Panacis Quinquefolii Radix decoction pieces [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(2): 328-336.
[14]	LIU Chang, YUAN Ding, ZHANG Ji-hong, HUANG Ling, HE Yu-min. Medicinal value of Lablab Semen Album and its seed coat based on HPLC fingerprint and chikusetsusaponin IVa content analysis [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(12): 2284-2292.
[15]	ZHENG Shu-ning, QI Hong, LIN Man-yi, ZHOU Yan-jun, ZHANG Ying, WU Meng-hua, CAO Hui, MA Zhi-guo. UPLC fingerprints and content determination of six components of Citri Fructus and its adulterants [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(11): 2018-2027.