Preparation of Anti-oxidation Bioactive Peptide of Rice Bran Protein

doi:A

Abstract

Abstract: This study screened the most suitable prolease of preparation of bioactive peptide with the indexes of Hydrolysis (DH%) and the eliminating rate of DPPH free radical. In order to find the most suitable zymohydrolysis condition of prolease, effects of concentration of substrate, pH, addition of protease and dissociation-time on the hydrolysis (DH%) and the eliminating rate of DPPH freeradical in hydrolyzing were investigated. Based on the single-factor experiment, the optimum conditions of extraction of bioactive peptide from rice bran by the Box-Behnken response surface methodology design of Design-Expert 7.0 were determined as follows: addition amount of enzyme 13970.82 U/g, dissociation-time 3 h and concentration 4.97%. The results showed that degree of hydrolysis of rice bran protein was 23.67% and the eliminating rate of DPPH free radical could reach 64.26% under the optimum conditions.

Key words: bran protein, degree of hydrolysis, DPPH radical scavenging abilities, response surface

CLC Number:

TS201.4

ZHOU Mei, ZHANG Min. Preparation of Anti-oxidation Bioactive Peptide of Rice Bran Protein[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(6): 793-.

References

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