Abstract: The influencing factors on the accumulation of theaflavins were systematically selected and optimized by response surface analysis.The effects of related factors were evaluated firstly by the Plackett-Burman method,and the amount of enzyme,reaction temperature,pH was identified as significant factors;the maximum cumulative concentration regions of theaflavins were then obtained through steepest ascent optimization algorithm,and the optimal catalytic conditions were finally established by the central composite and response surface methodology.The optimal processes were as follows:temperature 29.5 °C,pH 4.9,enzyme concentration 14400 U.Under the optimized processes,the accumulated concentration of theaflavins reached to 15.1 mg/mL.

Key words: theaflavins, fermentation, response surface methodology