Abstract: To Compare the anti-osteoporosis activity and the contents of total saponins and total flavonoids from different parts of Eclipta prostrata L..The effects of ethanol extracts from different parts of E.prostrata on the proliferation and differentiation of UMR106 cells were evaluated by the MTT method and measuring the activity of alkaline phosphatase (ALP) by p-nitrophenyl phosphate disodium method.The contents of total saponins and total flavonoid from different parts of E.prostrata were used to determine by Ultraviolet spectrophotometry method.The results show that the extracts of the root and leaf from E.prostrata stimulated UMR106 cells proliferations, the proliferation rate was 7.7% and 16.9% respectively at a concentration of 50 μg/mL,while the stem did not stimulate UMR106 cells proliferation.all of the extracts from root,stem and leaf of E.prostrata could increased the ALP activity respectively by 18.9%,20.1%,and 17.0% at a concentration of 100 μg/mL.The contents of total saponin and total flavonoids from the root,stem and leaf of E.prostrata were 3.6%,4.0%,2.2% and 1.4%,1.4%,3.4%,respectively.In Conclusion,all of the extracts from different parts of E.Prostrata have antiosteoporotic activity.The contents of total saponins and total flavonoids from different parts of E.prostrata have large difference.

Key words: Eclipta prostrata L., UMR106 cells, osteoporosis, total saponins, total flavonoids