Abstract: To explore the intervention effect of Huayu Tongluo traditional Chinese medicine on diabetic nephropathy (DN) rats and its effect on kidney fibronectin and laminin expression.Ten rats were randomly selected as normal control group (C group) from 55 male Sprague-Dauley rats,and the remaining rats were established by high-fat diet combined with low-dose streptozotocin intraperitoneal injection.Successful model rats were randomly divided into four groups,namely:model group (M group),Huayu recipe treatment group (H group),Tuolong recipe treatment group (T group),Irbesartan group (I group).Each treatment group gave corresponding drug lavage,once daily for 16 weeks.At the end of the 16^th week,The FBG,24 h UTP,PT,Scr,TG were tested.The renal tissues of rats were separated for pathological observation.The expression of FN and LN in renal tissue was tested by immunohistochemistry.Compared with control group,the FBG,24 hUTP,PT,TG and Scr levels of model group were significantly increased (P<0.05),the expression of FN and LN were significantly increased (P<0.05);compared with model group,the 24 hUTP,PT,TG levels of every treatment group were significantly reduced (P<0.05),the expression of FN and LN were significantly reduced (P<0.05).These results suggested that Huayu Tongluo traditional Chinese medicine showed a protective effect in the DN rats kidney,suggesting its therapeutic effect on DN rats may be related to the regulation of lipid metabolism,correction of blood hypercoagulable state,reduction of FN and LN expression and delay renal fibrosis.

Key words: diabetic nephropathy, Huayu Tongluo traditional Chinese medicine, FN, LN, renal fibrosis