Abstract: In this study,the content of hypaphorine from Vaccaria segetalis determined by high performance liquid chromatography(HPLC) was used as an evaluation index,single factor and orthogonal tests were carried out to optimize of water bath reflux extraction and ultrasonic extraction of hypaphorine from V.segetalis separately.The results showed the optimal parameters for the extraction of hypaphorine from V.segetalis by ultrasonic extraction were:liquid-solid ratio of 10:1 mL/g,60% ethanol as extraction solvent,350 W as ultrasonic power,80 ℃ as extraction temperature and 40 min as extraction time.Under these conditions,the content of hypaphorine from V.segetalis was 0.395 mg/g.The optimal water bath reflux extraction conditions were:ethanol concentration of 75%,extraction temperature of 95 ℃,extraction time of 2 h and liquid-solid ratio of 12:1 mL/g,the content of hypaphorine from V.segetalis was 0.345 mg/g.The efficiency,stability and repeatability of ultrasonic extraction were better than water bath reflux extraction.

Key words: Vaccaria segetalis, hypaphorine, water bath reflux extraction, ultrasonic extraction, extraction process