Abstract: Eight compounds were obtained from the fermentation of the polar-derived fungus Geomyces sp.3-1.The extract was isolated and purified by column chromatography on silica-gel,reversed phase chromatography,Sephadex LH-20 and HPLC.The structures of compounds were elucidated by physical and chemical properties and spectral analysis,and comparison with the data of literatures.The eight compounds were identified as paulownin(1), demethylincisterol A3(2),ergosta-7,22-dienen-3,6-dione(3),citreoanthrasteroid B(4),19-norergosta-5,7,9,22-tetraene-3β-ol(5),(22E)-5α,8α-epidioxyergosta6,22-dien-3β-ol(6),(3β,5α,6β,22E)-6-methoxyergosta-7,22-diene-3,5-diol(7) and ergosta-7,22-dien-3β-ol(8).Compounds 2-7 were firstly isolated from Geomyces species,and compound 2 was shown to have antibacterial and cytotoxic activities.

Key words: polar-derived fungus, Geomyces sp., secondary metabolites, biological activity