Abstract: The aim of the present study was to investigate the effect of Hawthorn flavonoidson for myocardial ischemia rats induced by isoproterenol,and subsequently discuss the different metabolic pathway.¹H NMR was used to exam the urine metabolites of rats,the pattern identification were adapted to revealed potential biomarkers in urine.There were significant differences in urine metabolies among the groups,six urinary metabolic products were compareded with the control group.After the treatment of hawthorn flavonoids (0.276 g/kg),the levels of DMA,DMG,Hippurate and citrate decreased (P < 0.05),TMAO and Creatinine increased (P < 0.05).Myocardial Ischemia Rats showed significant difference metabolic profile compared with the normal group.Hawthorn flavonoids (0.276 g/kg) changed the myocardial Ischemia metabolic profile to the normal status in some extent possibly through its regulation on the oxidative stress,calcium overload of cells,tricarboxylic acid cycle as well as the renal function.

Key words: metabolomics, myocardial Ischemia, hawthorn flavonoids, urine