Abstract: In this paper,the effects of curcumin on the histomorphology,ultrastructure,protein and gene expression of inflammatory factors in the kidney of rats with exercise-related renal ischemia-reperfusion injury was studied.72 SPF male Wistar rats at the age of 7-week-old were divided into 4 groups randomly:control group (C),general training group (M),overtraining group (OM),curcumin+overtraining group (COM).The rats in OM,COM groups were assigned under progressively increasing load training for 8 weeks，while the rats of COM group were given 200 mg/kg/d of curcumin in the volume as 5 mL/kg，other groups were given the same volume of 0.5% sodium carboxymethyl cellulose.Kill the rats 24 h after the last training，the renal tissue morphological was conducted by optical and electronic microscopy；serum creatinine and blood urea nitrogen was measured.Protein and gene of IL-1β,TNF-α and IL-6 in renal tissue were detected.The results showed:after progressively increasing load training for 8 weeks，renal tissue structures of C group and M group appear normal in pathology slice;histopathological abnormalities appear in OM group;the pathological changes of kidney tissue in COM group were significantly less than those in OM group.Paller score of OM group was significantly higher than that in C group,COM group was significantly lower than that of OM group.Under electron microscope,the structure of C group were normal;that of M group change slightly;OM group change obviously,and the pathological changes of COM group was significantly reduced compared to M group.Blood urea nitrogen and serum creatinine of OM group were significantly higher than those of C group,and those two in COM group were significantly lower than in OM group;IL-1β,TNF-α and IL-6 protein of ranal tissue in OM group was significantly higher than that in C group,and those proteins in COM group was significantly lower than those of OM group;for the expression of OM group，renal IL-1β mRNA,TNF-α mRNA and IL-6 mRNA were significantly higher than those in C group,but in COM group were lower than in OM group.Serum testosterone / corticosterone ratio in group OM was significantly lower than that in group C.The 8 weeks progressively increasing-load swimming training resulted in overtraining and exercise-induced renal ischemia-reperfusion injury in rats, accompanied by ultrastructure and pathological changes in the kidney.Curcumin can significantly reduce renal dysfunction and kidney tissue ultrastructure of the extent of the damage and the histopathological changes,which may be achieved by inhibiting the expression of inflammatory cytokines IL-1β,TNF-α and IL-6,resistance to various stress stimuli,as a result,curcumin can protect the rats from exercise-related renal ischemia-reperfusion injury,effectively improved the renal function,and alleviated the morphological and ultrastructural changes.

Key words: Curcumin, Overtraining, exercise-induced ischemia-reperfusion, inflammatory factors