Abstract: To evaluate the best anti-inflammatory active sites of Phyllanthus emblica extracts.In this study,different solvent extracts were obtained by using water,ethanol,ethyl acetate and petroleum ether as extraction solvent.The model of inflammation was induced by lipopolysaccharide-induced RAW264.7 macrophages.The NO secretion and cytokine release （TNF-α、IL-1β、IL-6）were used as indicators to select part of the best anti-inflammatory activity of Phyllanthus emblica.The different solvent extracts of Phyllanthus emblica had no obviously cytotoxicity (P>0.05) at the concentration of 25~400 μg/mL.Compared with the model group,water①,water② and ethanol extract could significantly inhibit the secretion of NO from macrophages induced by LPS (P<0.05).In the cytokine test,the ethanol extract significantly inhibited the secretion of IL-1β and TNF-α (P<0.01) by LPS-induced RAW264.7 macrophages.The ethyl acetate extract inhibited LPS-induced RAW264.7 macrophage secretion of IL-6 is the best.Phyllanthus emblica ethanol extract was screened as the best anti-inflammatory active site which significantly inhibited the secretion of NO and cytokines (TNF-α,IL-1β,IL-6) in RAW264.7 macrophages and it can be used as a target for the subsequent separation of the anti-inflammatory active substance monomer.

Key words: Phyllanthus emblica, extract, anti-inflammatory, NO, cytokine