Abstract: Seventeencompounds were isolated from the EtOAc extract of endophytic fungus Stagoosporopsis oculihominis in Dendrobium huoshanense by chromatography technology and their structures were identified as acetyl-L-isoleucine (1),N-(4-oxopentyl)acetamide (2),N_b-(2-(1H-indol-3-yl)ethyl)acetamide (3),uracil (4),1H-indole-3-carboxylic acid (5),2-(1H-indol-3-yl)acetic acid (6),adenine nucleoside (7),Cyclo-(L)-proline-(D)-leucine (8),Cyclo-(L)-proline-(L)-leucine (9),Cyclo-(L)-phenylalanine-(L)-leucine (10),oleic acid (11),α-linoleic acid (12),8(E)-hexadecenoic acid (13),linolenic acid (14),α-linoleate (15),22E-7αmethoxy-5α,6α–epoxyergosta-8(14),22-dien-3β-ol (16),demethylincisterol (17)on the basis of comprehensive spectroscopic analysis.All these compounds are isolated from Stagonosporopsis oculihominis for the first time,including that compound 1 was a new natural product.Compounds 2,16 and 17 exhibited cytotoxicactivities,especially,compound 17 showed moderate cytotoxic activities with IC₅₀ values of 39.2~53.2 μM.

Key words: Dendrobium huoshanense, Stagonosporopsis oculihominis, endophytic fungi, secondary metabolites