The plant of Murraya panaculata (L.) Jack was powered,extracted with ethyl acetate.The ethyl acetate extract was separated by silica gel column chromatography,preparative high performance liquid chromatography (HPLC) and recrystallization methods,then six compounds have been isolated.On the basis of nuclear magnetic resonance (NMR) and comparison of their spectra with values reported in the literature,the compounds designated as 5,7,8,3′,4′-pentamethoxyflavone (1),5,7,8,3′,4′,5′-hexamethoxyflavone (2),5,7,3′,4′,5′- pentamethoxyflavone (3),5,6,7,3′,4′,5′-hexamethoxyflavone (4),5-hydroxy-6,7,3′,4′-tetramethoxyflavone (5) and 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone (6).Compound 1 was isolated from the Murraya species and M. panaculata for the first time.The DPPH radical scavenging experiments of compounds showed that all of six compounds had antioxidant activity,among which 5-hydroxy-6,7,3′,4′-tetramethoxyflavone (5) and 5-hydroxy-6,7,8,3′,4′-pentamethoxyflavone (6) had stonger antioxidant activity.