To investigate preventive effect of total flavonoids from Nymphaea candida (NCTF) on carbon tetrachloride (CCl₄) -induced liver fibrosis in mice.Seventy-two male KM mice were randomly divided into normal group,model group,colchicine group (0.1 mg/kg),and NCTF group (50,100,200 mg/kg).Except normal group,other groups were intraperitoneally injected with 10% CCl₄ olive oil solution twice a week.The day after animals modeling,other groups were given intragastric administration for 8 weeks according to preset dose except the model group and normal group.Sixteen hours after the last medication,the eye balls of the mice were removed,blood taken;the mice were then killed,liver and spleen were collected.Pathological changes of liver tissue were observed by HE and Masson staining.Serum AST,ALT,and ALP levels were measured by a fully automatic biochemical analyzer;ELISA assay was used to determine serum HA,LN,PCIII,IV-C,IL-6 and TNF-α contents;Liver tissue Hyp content was detected according to hydroxyproline kit.IKKα,IκBα and NF-κB p65 mRNA expression were detected by fluorescence quantitative PCR.Compared with model group,the serum AST,ALT,ALP,LN,HA,PCIII and IV-C levels were significantly decreased by NCTF (P<0.05),and pro-inflammatory cytokine IL-6 and TNF-α levels were decreased (P<0.05).Liver tissue Hyp level was also significantly reduced (P<0.05).In addition,compared with model group,NCTF can significantly reduce IKKα,IκBα and NF-κB p65 mRNA expression (P<0.05).These results suggest that NCTF have better anti-hepatic fibrosis effect,and its mechanism may be related to reduce cell interstitial collagen deposition,decrease proinflammatory factor expression and regulate NF-κB signaling pathway.