Flavonoids from the seeds of Brucea javanica and their anti-inflammatory and anticomplement activities were studied.The compounds were isolated and identified by chromatographic and spectroscopic methods.The anti-inflammatory and cytotoxic activities of the compounds were determined by Griess method and CCK-8 method.Determination of CH₅₀ activity of classical complement pathway of flavonoids by hemolysis method.Compounds (1-12) obtained from the ethyl acetate fraction of the ethanol extract were identified as luteolin (1),apigenin (2),luteolin-7-O-β-D-glucoside (3),7-methoxy-luteolin (4),quercetin-3-O-α-L-rhamnoside (5),luteolin-3′-O-β-D-glucoside (6),kaempferol-3-O-α-L-rhamoside (7),apigenin-7-O-β-D-glucoside (8),3′-methoxy-luteolin (9),apigenin-7-O-neohesperidoside (10),rutin (11) and isovitexin (12).Compounds 1,2,4,6,7,8,11 and 12 were obtained from this species for the first time.The results of screening demonstrated that compounds 1,2,3,4,5,9 and 11 showed moderate NO inhibitory effects,while all the compounds,except for compounds 4 and 9,possessed anticomplement activity and exhibited well structure-activity relationship.