In order to establish a more comprehensive quality evaluation method of Cyathula officinalis Kuan.The water content,total ash,water-cooled extract and ethanol cold extract were determined according to the Pharmacopoeia of the peoples Republic of China (2015);A new HPLC method was established for the determination of three components of cyasterone precyasterone and oleanolic acid in C. officinalis.custer analysis and principal component analysis were carried out by SPSS 200 software.This study comprehensively evaluated the quality of C. officinalis from 54 places in China,the water content,total ash,extract and content of 54 batches of C.officinalis samples all meet the pharmacopoeia standard,provided a basis for the further development of C. officinalis.