The objective of this study was to provide scientific supports for the utilization and development of Lepidium latifolium L. by setting up its quality control specification.In accordance with the methods of Chinese Pharmacopoeia,the crude drug samples collected from 12 different producing areas were used to determine the contents of extract,ash and moisture.The qualitative identification was determined by thin layer chromatography (TLC) method.The content of quercetin-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranosyl was determined by high performance liquid chromatography (HPLC) method.According to the study,the total amount of ash in the samples should not exceed 12%,the alcohol extract should not less than 10%,and the moisture content should not more than 7%.The standard showed good linear relationship within its own range (r ≥0.999 4),whose average recovery was 98.79% with the RSD of 2.16%.The TLC spots were clear with good separation.The precision,stability and repeatability of quercetin-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranosyl  were less than 2%.In conclusion,the established method can be used for the quality control of Lepidium latifolium L.