To establish an analysis method of Yupingfeng Powder based on HPLC fingerprint combined with chemometrics analysis,and determined the contents of 11 components in it,which provides the scientific basis for quality control of Yupingfeng Powder.The chromatographic column was Shimadzu Inertsil ODS-2 C₁₈ (250 mm× 4.6 mm,5 μm),the mobile phase was composed of the acetonitrile-water solution in gradient elution manner at a flow rate of 1.0 mL/min,the column temperature was set at 30 ℃,the detection wavelength was set at 230 nm.The HPLC fingerprint of 15 batches of Yupingfeng Powder was established,the similarity was more than 0.83,24 common peaks were calibrated and 11 chromatographic peaks were identified.They are prim-O-glucosylcimifugin,calycosin-7-O-β-D-glucoside,cimifugin,5-O-methylvisammioside,sec-O-glucosylhamaudol, calycosin, formononetin, ononin, astragaloside III,astragaloside I,astragaloside II,respectively.The results of content determination were 0.104-0.449,0.048-0.121,0.047-0.111,0.129-0.550,0.017-0.037,0.025-0.053,0.030-0.118,0.009-0.025,0.148-0.615,0.088-0.382,0.073-0.385.The results were analyzed by using SPSS 23.0,SIMCA-P 13.0,and other software to perform HCA,PCA,and OPLS-DA,and other chemometric analyses of 15 batches of Yupingfeng Powder,fifteen batches of samples were grouped into two groups.The established quality evaluation method of Yupingfeng Powder is stable and reproducible and can be used for the quality control and evaluation of Yupingfeng Powder.