To investigate the chemical constituents and biological activities from the fruits of Lycium barbarum L.,seventeen compounds were isolated and purified from the ethyl acetate extract of 75% ethanol extract of fruits of L. barbarum by using various chromatographic methods,their structures were elucidated via NMR,MS techniques as:oxypeucedanin hydrate (1),pangelin (2),byakangelicol (3),phellopterin (4),3-［2-formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl］ pentanedioic acid (5),4-［2-formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl］butanoic acid (6),3-methoxyl-1H-pyrrole (7),nicotiamide (8),5-hydroxyl-2-hydroxymethylpyridine (9),5-hydroxymethyl-furaldehyde (10),stigmast-4-en-3-one (11),(+)-hannokinol (12),trans-ferulic acid (13),4-hydroxycinnamic acid (14),2,4-dimethoxy-2-methyl-(6H)-pyran-3-one (15),4-hydroxybenzaldehyde (16),p-hydroxybenzoic acid (17).Among these compounds,1-4 were isolated from Lycium genus for the first time,and 11 was isolated from L. barbarum for the first time.The biological activities were investigated through DPPH radical scavenging and tyrosinase-catalyzed L-dopa oxidation effects.The results indicated that compounds 1,4,5,12,14 and 15 showed potent scavenging ability on DPPH free radical with IC₅₀ values ranging from 0.17 μg/mL to 87.56 μg/mL,and 3-9,11,14 and 17 exhibited good inhibition on tyrosinase with IC₅₀ values range of 1.65-68.76 μg/mL.Among them,the antioxidant capacity of 5 and 14 was better than the positive control ascorbic acid,and the inhibitory capacity of 3,6,8 and 11 was higher than arbutin(positive control).

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