NATURAL PRODUCT RESEARCH AND DEVELOPMENT ›› 2020, Vol. 32 ›› Issue (12): 2094-2100.doi: 10.16333/j.1001-6880.2020.12.014

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Anticomplement and anti-inflammatory flavones from the seeds of Brucea javanica

HE Xiao1,GUO Wen-jing1,WU Jia-hui1, XIONG Zi-wei1,TAN Ting1,FENG Yu-lin1,2,YANG Shi-lin2,WEN Quan1*   

  1. 1Jiangxi University of Traditional Chinese Medicine;2State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment,Nanchang 330006,China

  • Online:2020-12-28 Published:2020-12-24


Flavonoids from the seeds of Brucea javanica and their anti-inflammatory and anticomplement activities were studied.The compounds were isolated and identified by chromatographic and spectroscopic methods.The anti-inflammatory and cytotoxic activities of the compounds were determined by Griess method and CCK-8 method.Determination of CH50 activity of classical complement pathway of flavonoids by hemolysis method.Compounds (1-12) obtained from the ethyl acetate fraction of the ethanol extract were identified as luteolin (1),apigenin (2),luteolin-7-O-β-D-glucoside (3),7-methoxy-luteolin (4),quercetin-3-O-α-L-rhamnoside (5),luteolin-3′-O-β-D-glucoside (6),kaempferol-3-O-α-L-rhamoside (7),apigenin-7-O-β-D-glucoside (8),3′-methoxy-luteolin (9),apigenin-7-O-neohesperidoside (10),rutin (11) and isovitexin (12).Compounds 1,2,4,6,7,8,11 and 12 were obtained from this species for the first time.The results of screening demonstrated that compounds 1,2,3,4,5,9 and 11 showed moderate NO inhibitory effects,while all the compounds,except for compounds 4 and 9,possessed anticomplement activity and exhibited well structure-activity relationship.

Key words: Brucea javanica , (L.) Merr, flavonoid, anti-inflammatory activity, anticomplement activity

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