The key pathogenic genes of liver cancer were screened by bioinformatics methods to explore their expression level,pathogenesis,immune infiltration,mutation and prognosis,and to further explore the potential traditional Chinese medicine for targeted treatment of liver cancer.The differentially expressed genes (DEGs) in normal and hepatoma tissues were analyzed by data set GSE19665;Go and KEGG analysis were performed on DEGs to construct the interaction network of DEGs protein and screen the key genes of liver cancer;Mutation,immune infiltration,expression and prognosis of key genes were analyzed;Excavate potential traditional Chinese medicine and natural active ingredients for the treatment of liver cancer.159 significant DEGs were screened,which were mainly involved in immune and inflammatory responses and involved in p53,mTOR and other signal pathways;Ten key genes were screened out.The expression of four genes such as CDC20 in HCC was significantly increased,and the overall survival time of patients in the high expression group was significantly reduced;The overall survival and disease-free survival of patients with key gene mutations were significantly reduced;The expression of key genes was closely related to the infiltration of immune cells;36 kinds of traditional Chinese medicine and 105 kinds of active ingredients were screened by the key gene target.In this study,the key pathogenic genes of liver cancer were identified,and their abnormal expression was closely related to the poor prognosis and immune cell infiltration of liver cancer.The potential traditional Chinese medicine and its active ingredients for the treatment of liver cancer were excavated.The research results can provide a scientific basis for the diagnosis,treatment and prognosis of liver cancer.