Abstract: Acute toxicity test,three genetic toxicity tests and 30 days oral toxicity test were carried out to evaluate the toxicological safety of total flavones extract from white safflower variety (TFES).Mice acute toxicity test showed the LD₅₀ of TFES was higher than 10.0 g/kg·BW.TFES belonged to actually non-toxic substances according to the acute toxicity dosage classification.Three genetic toxicity tests including Ames,Mice Bone Marrow Cell Micronucleus and Mice Sperm Abnormality showed negative results.Rats 30 days oral toxicity test of TFES showed no evident poisoning symptom and no death.There was no significant difference between different dosage groups of TFES and control group on animal weight,food utility,hematology,bio-chemistry,organ/body weight ratio and several indexes of pathological organization.Therefore,TFES had no toxicity which provided a secure basis for its further development and application.

Key words: white safflower variety, total flavones, acute toxicity, hereditary toxicity, 30 days oral toxicity test