天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (1): 103-108.doi: 10.16333/j.1001-6880.2015.01.022

• 开发研究 • 上一篇    下一篇

响应面法优化超声辅助提取贯众多酚工艺

陆洋,彭飞,黄丽霞,习伟佳,马吉福,马骥*   

  1. 陕西师范大学生命科学学院 教育部药用植物资源及天然药物化学重点实验室,西安 710119
  • 出版日期:2015-01-31 发布日期:2015-02-12

Optimization of Ultrasonic-assisted Extraction Conditions of Polyphenol from Cyrtomium fortunei by Response Surface Methodology

LU Yang, PENG Fei, HUANG Li-xia, XI Wei-jia, MA Ji-fu, MA Ji*   

  1. Life Science College,Shaanxi Normal University,Key Laboratory of Ministry of Education for Medicinal  Plant Resource and Natural Pharmaceutical Chemistry,Xi'an 710119,China
  • Online:2015-01-31 Published:2015-02-12

摘要: 利用响应面法优化贯众多酚的提取工艺。在单因素试验的基础上,选择超声时间、超声温度、料液比、乙醇浓度为自变量,贯众中多酚的提取率为响应值,进行Box-Benhnken中心组合实验设计,运用响应面法优化提取条件,得到最优提取条件如下:超声时间20 min,超声温度46 ℃,料液比1:40,乙醇浓度35%,经验证,贯众中多酚的提取率为8.11660%,理论值为8.12986%,RSD为0.15%。该工艺高效、稳定,有一定的应用价值。

关键词: 贯众, 多酚, 超声提取, 响应面法

Abstract: The objective of this study was to optimize the extraction of polyphenols from Cyrtomium fortunei through response surface methodology (RSM).Based on single factor experiments,ultrasonic time,ultrasonic temperature,solid-liquid ratio and ethanol concentration were selected to design Box-Behnken central composite.By means of regression analysis,the results showed that the optimal conditions to extract polyphenols from C.fortunei were as follows:ultrasonic time of 20 min,ultrasonic temperature of 46 ℃,solid-liquid ratio of 1:40 and ethanol concentration of 35%.Under these conditions,the extraction yield of polyphenols reached 8.11660%.Compared with the theoretical value (8.12986%),the RSD was 0.15%.The result indicated that the extraction system was efficient,reliable and practical.

Key words: Cyrtomium fortunei, polyphenols, ultrasonic-assisted, response surface methodology

中图分类号: 

TS201