关键词: 真菌, 发酵, 锡尾矿, 次生代谢产物

Abstract: The secondary metabolites of a fungus isolated from tin mine tailings were separated by silica gel column,sephadex LH-20 gel column and HPLC.14 compounds were obtained.Their structures were identified by mass spectrometry and nuclear magnetic resonance analyses and elucidated as:Stachyflin (1),Brevicompanine E(2),Brevicompanine H (3),Brevicompanine D (4),(2S,3S)-3,5-dihydroxy-2-((Z)-4-(hydroxymethyl)-8- methylnona-3,7-dien-1-yl)-2-methyl-3,4,8,9-tetrahydropyrano［2,3-e］isoindol-7(2H)-one (Stachybotrin A) (5) 5-((1R,2R,5R,6R)-2,5-dihydroxy-7-oxabicyclo［4.1.0］heptan-2-yl)-3-((1S,2S,4aR,6S,8aS)-2,6-dimethyl1,2,4a,5,6,7,8,8a-octahydronaphthalene-1-carbonyl)-4-hydroxypyridine-2(1H)-one(Apiosporamide) (6),(3s)-6,8- dihydroxy-3-methyl-3,4-dihydro-1H-isochromen-1one (7),Adenosine (8),4-hydroxybenzaldehyde (9),methyl (5-amino-2-hydroxyphenyl) acetate (10),Uridine (11),Acetylstachyflin (12),Nicotinamide (13),(S)-7-benzyl -6-methyl-6,7-dihydrobenzo［6,7］［1,4］diazepino［2,1-b］quinazoline-5,13-dione(Benzomalvin A) (14).Compound 5 showed antitumor activity with ED₅₀ values of 21.59,23.45 and 37.36 μg/mL against HT-29,A-549 and MCF-7,respectively.

Key words: fungus, fermentation, tin mine, secondary metabolites