天然产物研究与开发 ›› 2015, Vol. 27 ›› Issue (Suppl): 87-90.doi: 10.16333/j.1001-6880.2015.S.022

• 研究简报 • 上一篇    下一篇

油茶组织培养研究

颜凤霞,田凡,侯娜,王港,文弢,王莲辉*   

  1. 贵州省林业科学研究院,贵阳 550005
  • 出版日期:2015-12-01 发布日期:2016-01-12

Tissue Culture Technology of Camellia oleifera

YAN Feng-xia,TIAN Fan,HOU Na,WANG Gang,WEN Tao,WANG Lian-hui*   

  1. Guizhou Academy of Forestry,Guizhou Guiyang 550005,China
  • Online:2015-12-01 Published:2016-01-12

摘要: 为了筛选最适合油茶诱导腋芽生长、分化及油茶生根的培养条件及试管苗移栽基质,以油茶带腋芽的茎段为外植体,通过不同激素组合,激素配比等方式进行试验。结果表明:油茶腋芽诱导培养基为MS,茎段在含有MS+2.0 mg/L6 BA+ 0.1 mg/LNAA+3%糖+0.4 %琼脂的培养基中芽的增殖能力较强;适宜的生根培养基为MS+2.0 mg/L IBA+3%糖+0.4 %琼脂,适宜试管苗移栽基质为河沙:珍珠岩=2:1,移栽成活率达98%。

关键词: 油茶, 组织培养, 培养条件

Abstract: In order to select the culture conditions of the most suitable induced lateral bud growth,differentiation and differential rooting,taking stem with axillary bud as explant,tested through different hormone combinations and hormone proportion.The results were as follows:the suitable induced lateral bud growth was MS + sucrose 30 g/L + Agar 4.0 g/L,and its suitable differentiation medium was MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L + sucrose 30 g/L + Agar 4.0 g/L,the differential rooting was MS+2.0 mg/L IBA+ sucrose 30 g/L + Agar 4.0 g/L.The substrate of suitable tube seedlings transplanting was a mixture of river sand and perlite,ratio of 2:1,transplanting survival rate was 98%.

Key words: Camellia oleifera, tissue culture, culture conditions

中图分类号: 

S571.1