茶叶水提物对猪源耐药大肠杆菌的抑制作用

doi:10.16333/j.1001-6880.2016.12.019

天然产物研究与开发 ›› 2016, Vol. 28 ›› Issue (12): 1959-1964.doi: 10.16333/j.1001-6880.2016.12.019

茶叶水提物对猪源耐药大肠杆菌的抑制作用

杨建英1,2，郭小权3，肖莉春1,3，黄名钱1,3，孔祥峰1*

1 中国科学院亚热带农业生态研究所中国科学院亚热带农业生态过程重点实验室，长沙 410125；2 河南科技大学医学技术与工程学院，洛阳 471003；3 江西农业大学动物科学技术学院，南昌 330045

出版日期:2016-12-31 发布日期:2017-03-31

Inhibitory Effects of Tea Water Extracts on Antibiotic-resistant Isolates of Escherichia coli from Swine

YANG Jian-ying1,2,GUO Xiao-quan3,XIAO Li-chun1,3,HUANG Ming-qian1,3,KONG Xiang-feng1*

1 Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture, Chinese Academy of Sciences,Changsha 410125,China; 2 College of Medical Technology and Engineering, Henan University of Science and Technology,Luoyang 471003,China; 3 College of Animal Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China

Online:2016-12-31 Published:2017-03-31

摘要/Abstract

摘要： 本研究旨在考察茶叶水提物对耐药大肠杆菌的抑制作用，为大肠杆菌病的防治提供依据。采用体外抑菌试验测定红茶水提物和绿茶水提物对猪源耐药大肠杆菌分离株的抑制活性，然后观察具有较强抑菌活性的绿茶水提物对大肠杆菌攻毒小鼠的保护作用。结果表明，随着浓度的增加，两种茶叶水提物的抑菌活性增强；红茶水提物和绿茶水提物对13株大肠杆菌分离株的MIC值分别为62.5～250.0 mg/mL、62.5～125.0 mg/mL，提示绿茶水提物的抑菌活性较强；两种茶叶水提物对13个大肠杆菌分离株的抑制活性强弱表现出一定的差异。攻毒后48 h，攻毒组小鼠全部死亡，绿茶水提物组小鼠的存活率为20%；绿茶水提物组小鼠的十二指肠和空肠肠绒毛高度与对照组比较差异不显著，而显著大于攻毒组（P<0.05），提示绿茶水提物对大肠杆菌攻毒小鼠的肠道损伤具有一定缓解作用。上述结果提示，绿茶水提物对大肠杆菌具有很好的抑制作用，可作为防治大肠杆菌病的备选生物活性物质之一。

关键词: 茶叶水提物, 大肠杆菌, 抑菌活性, 保护作用

Abstract: This study was conducted to investigate the inhibitory effects of tea water extracts against antibiotic-resistant Escherichia coli (E.coli) in order to provide information for the prevention and treatment of colibacillosis.The inhibitory activities of water extracts from red tea and green tea against antibiotic-resistant E.coli isolates from swine were determined by in vitro antibacterial test.The protective effect of water extracts of green tea with stronger inhibitory activity on mice challenged by antibiotic-resistant E.coli were observed in vivo. Results showed that the inhibitory activities against E.coli of two water extracts of tea increased with the increasing of their concentrations.The minimum inhibitory concentrations of water extracts of red tea and green tea against 13 tested E.coli strains were 62.5-250.0 and 62.5-125.0 mg/mL,respectively,which suggested that the water extracts of green tea presented stronger antibacterial activity.Two water extracts of tea presented different antibacterial activities against different strains of E.coli. At 48h post-challenge,all mice in the challenged group were dead,while survival rate of mice in the water extracts of green tea group was 20%;the villus height of duodenum and jejunum of mice in the water extracts of green tea group had no significant difference compared with the control group,while was higher (P<0.05) compared with the challenged group,which suggested that the water extracts of green tea presented mitigative effects to intestinal trauma caused by E.coli challenge.These findings indicated that water extracts of green tea presented stronger inhibitory effects against antibiotic-resistant E.coli, which could be used as an alternative bioactive substance for the prevention and treatment of colibacillosis.

Key words: water extracts of tea, Escherichia coli;antibacterial activity;protective effects

中图分类号:

S816.7 S852.61

杨建英1,2，郭小权3，肖莉春1,3，黄名钱1,3，孔祥峰1*. 茶叶水提物对猪源耐药大肠杆菌的抑制作用[J]. 天然产物研究与开发, 2016, 28(12): 1959-1964.

YANG Jian-ying1,2,GUO Xiao-quan3,XIAO Li-chun1,3,HUANG Ming-qian1,3,KONG Xiang-feng1*. Inhibitory Effects of Tea Water Extracts on Antibiotic-resistant Isolates of Escherichia coli from Swine[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2016, 28(12): 1959-1964.

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茶叶水提物对猪源耐药大肠杆菌的抑制作用

Inhibitory Effects of Tea Water Extracts on Antibiotic-resistant Isolates of Escherichia coli from Swine

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