关键词: 安子合剂, 抗磷脂抗体, 流产, 母胎界面, TLR, 肿瘤坏死因子-&alpha

Abstract: The objective of the study was to elucidate the effect of Anziheji on TLR2,TLR4 in maternal fetal interface and Tumornecrosis factor-α (TNF-α) in antiphospholipid antibodies-positive abortive mice.The mice model immuned with human-β2-glycoprotein I (β-2GP I) was built to calculate embryo resorption rate,concentration of anti-β-2GP I antibody and TNF-α in peripheral blood were determined by ELISA.TLR2 and TLR4 mRNA expression level in placenta tissue was determined by real-time and quantitative PCR.The protein expression of TLR2 and TLR4 ws determined by immunohistochemical method.The results showed that,TLR2 and TLR4 mRNA level was obviously enhanced and proteins were in diffusely higher expression in model group compared with the blank group.Compared with model group,Anziheji evidently reduced embryo resorption rate as well as the concentration of anti-β-2GPⅠantibody and TNF-α.At the same time,mRNA level of TLR2 and TLR4 was down-regulated and TLR2 and TLR4 proteins were in lower expression.Above all,Anziheji inhibited key molecules of upstream in signal transduction pathways of TLR2 and TLR4 in maternal-fetal interface and reduced inflammation factor release,which may be one of its action mechanisms.

Key words: Anziheji, antiphospholipid antibody, abortion, maternal-fetal interface, TLR, TNF-&alpha