天然产物研究与开发 ›› 2017, Vol. 29 ›› Issue (11): 1905-1909.doi: 10.16333/j.1001-6880.2017.11.016

• 研究简报 • 上一篇    下一篇

高效液相色谱法同时测定青钱柳中9种指标成分的含量

汪荣斌1,2，秦亚东1，陈颖3，王存琴3，李林华1，周守标2*   

  1. 1安徽中医药高等专科学校；2安徽师范大学；3皖南医学院，芜湖 241000
  • 出版日期:2017-11-29 发布日期:2017-11-30
  • 基金资助:

    安徽省卫生计生委中医药科研课题（2014zy45）；安徽省高校优秀青年人才支持计划（gxyq2017209）；安徽高校自然科学研究重点项目（KJ2015A343）；安徽省高校学科（专业）拔尖人才学术资助重点项目（gxbjZD20161 06）

Simultaneous Determination of Nine Chemical Markers of Cyclocaryae Folium by HPLC

WANG Rong-bin1,2, QIN Ya-dong1, CHEN Ying3, WANG Cun-qin3, LI Lin-hua1, Zhou Shou-biao2*   

  1. 1Anhui college of Traditional Chinese Medicine,Anhui Wuhu 241000,China; 2Anhui Normal University,Anhui wuhu 241000,China; 3Wannan medical college,Anhui Wuhu 241000,China
  • Online:2017-11-29 Published:2017-11-30

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摘要： 建立高效液相色谱法同时测定青钱柳中绿原酸、香草酸、逆没食子酸、异槲皮苷、阿福豆苷、槲皮苷、山奈酚、山奈素和乌苏酸含量的方法。采用Agilent ZORBAX Eclipse XDB-C18色谱柱（150 mm×4.6 mm，3.5 μm）；流动相为0.2%磷酸乙腈-0.2%磷酸水，梯度洗脱；流速为0.7 mL/min，柱温35 ℃；检测波长：香草酸、逆没食子酸和乌苏酸为210 nm；绿原酸、异槲皮苷、阿福豆苷、槲皮素、山奈酚和山奈素为360 nm。青钱柳在该色谱条件下9种成分分离较好，平均加样回收率为95.86%~103.30%，RSD<2.5%。该方法简便、准确、重复性好，适用于青钱柳中绿原酸、香草酸、逆没食子酸、异槲皮苷、阿福豆苷、槲皮苷、山奈酚、山奈素和乌苏酸含量的同时测定。

关键词: 青钱柳, 高效液相色谱, 多指标成分, 不同产地, 含量测定

Abstract: HPLC method was developed for the simultaneous determination of chlorogenic acid,vanililic acid,ellagic acid, sioquercitin, afzelin, quercetin, kaempferol, kaempferidel and ursolic acid in Cyclocaryae Folium.The ZORBAX Eclipse XDB-C18 column (150 mm×4.6 mm,3.5 μm) was used with 0.2% phosphoric acid in acetonitrile (A) and 02% phosphoric acid in water (B) as mobile phases in gradient elution mode.The flow rate was 0.7 mL/min,the column temperature was 35 ℃.The DAD detection wavelength was set at 210 nm for vanililic acid,ellagic acid and ursolic acid,360 nm for the others.As a result,nine chemical markers were separated well,the average recoveries were between 9586-103.30%,RSD<2.5%.The developed HPLC method was simple,accurate and reproductive.It is suitable for the determination of nine chemical markers of Cyclocaryae Folium.

Key words: Cyclocaryae Folium, HPLC, multiple chemical marks, content determination

中图分类号: 

R284.1

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