关键词: 胰高血糖素样肽素-1, 糖基化终产物, 活性氧, 成纤维细胞, 凋亡, 细胞保护

Abstract: To investigate the effect of glucogon like peptide-1 on fibroblast cells against AGEs-induced apoptosis and potential molecular mechanisms.Fibroblast cells cultured in vitro were divided into the following five groups:normal control group,200 mg/L AGEs group,200 mg/L AGEs+5 nmol/L GLP-1 group,200 mg/L AGEs+10 nmol/L GLP-1 group and 200 mg/L AGEs+20 nmol/L GLP-1 group.Cell viability rate was measured by CCK-8 assay,ROS production was measured by DCFH-DA fluorescent probe;Cells in different groups were stained with Annexin V-FITC/PI and then apoptosis rate was detected by flow cytometry;Nucleus morphology was observed under fluorescence microscope after being incubated with Honchest 33258;Western blot was applied to assess the apoptotic components expression including Caspase-3 and Bcl-2.compared with control group,cell viability rate in AGEs group were decreased,repectively,in a dose-dependent manner;cell apoptosis and ROS production in fibroblast cells were remarkably increased in AGEs group;However,compared with AGEs group,GLP-1 reduced ROS production and ameliorated cell apoptosis caused by AGEs;the expression of pro-apotosis proteins Caspase-3 was decreased,the expression of anti-apoptosis proteins like Bcl-2 was increased.Hence,GLP-1 protected fibroblast cells against AGEs-induced apoptosis,which was possibly related to the reduction of the active oxygen.

Key words: GLP-1, AGEs, ROS, fibroblast cells, apoptosis, cell preservation