天然产物研究与开发 ›› 2019, Vol. 31 ›› Issue (3): 482-488.doi: 10.16333/j.1001-6880.2019.3.018

• 开发研究 • 上一篇    下一篇

酸酯碱成分结合指纹图谱优选盐黄柏炮制工艺

程中琴1，刘小妹1，施崇精1，王姗姗1，盛蓉2*，袁强华2，宋英2   

  1. 1成都中医药大学药学院，成都 610075；2成都中医药大学附属医院，成都 610072
  • 出版日期:2019-04-02 发布日期:2019-04-02
  • 基金资助:

    国家中药标准化项目-黄柏（ZYBZH-Y-SC-H）

Acids,alkaloids and ester combined with HLPC fingerprint to optimize the processing of stir-frying with salt-water for Phellodendron chinense Schneid bark

CHENG Zhong-qin1,LIU Xiao-mei1,SHI Chong-jing1,WANG Shan-shan1,SHENG Rong2*,YUAN Qiang-hua2,SONG Ying2   

  1. 1Pharmacy college,Chengdu University of TCM,Chengdu 610075,China; 2The Affiliated Hospital of chengdu University of TCM,Chengdu 610072,China  
  • Online:2019-04-02 Published:2019-04-02

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摘要： 通过测定三大类成分含量，结合盐黄柏指纹图谱优选盐黄柏炮制工艺。采用HLPC-DAD法测定盐黄柏中6种生物碱以及2种酸类、1种内酯，共9种，3大类成分含量。以AHP法确定各指标成分权重，综合评分结合指纹图谱，通过单因素及正交设计实验，定性定量的分析炮制温度、炮制时间、炒药量对盐黄柏炮制的影响，优选盐黄柏炮制工艺。优选炒制工艺为：180 ℃，20 min，300 g药材（占容器体积1/4）。三大类成分结合指纹图谱优选的盐黄柏炮制工艺稳定、可行；此外，该方法可用于类似工艺的筛选。

关键词: 黄柏, 盐炙, 酸类, 生物碱类, 酯类, 指纹图谱

Abstract: To research the HPLC fingerprint and three major chemical components changes of Cortex Phellodendri,HPLC.was ued to determine three kinds compositions in Phellodendron chinense Schneid bark,including six alkaloids,two acids and an ester.AHP was adopted to determine the weight of each index.Comprehensive score combined with HLPC Fingerprint used to optimize the Processing of Stir-bake with Salt-water for Phellodendron chinense Schneid by the design of single factor and orthogonal test.Qualitative and quantitative analysis of the effects of processing temperature,processing time and the amount of fried.The comprehensive study of HLPC fingerprint and three major chemical components to optimize the processing of stir-bake with salt-water for Phellodendron chinense Schneid bark was as following:180 ℃,20 min,300 g (volume 1/4 of container).The processing technology of Phellodendron chinense bark was stable and feasible optimized by three kinds of ingredients combined with HLPC fingerprints,can be used for mass production.In addition,this method can be used for screening similar processes.

Key words: Phellodendron chinense Schneid, stir-bake with dalt-water, alkaloids, acids, ester, HLPC fingerprint

中图分类号: 

R932

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